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• W2019753453 abstract "Transgenic rice (Oryza sativa L.) cell lines engineered with the pea diamine oxidase cDNA in antisense orientation under the control of two different promoters were recovered using particle bombardment. Plasmids p35Sdaoa and pEdaoa contained the pea diamine oxidase cDNA driven by the CaMV35S and the pea ENOD12 nodulin promoter, respectively. Molecular analyses confirmed the stable integration of the transgene and active transcription (mRNA) with both promoters driving the gene of interest. We observed a 2-fold decrease in diamine oxidase activity (P 0.05) was observed in spermine levels in pEdaoa transformants. We demonstrate for the first time the down-regulation of an endogenous enzyme involved in polyamine metabolism in plants accompanied by a concomitant increase in the concentration of putrescine and spermidine." @default.
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• W2019753453 date "2000-10-01" @default.
• W2019753453 modified "2023-09-27" @default.
• W2019753453 title "Transgenic cell lines as a useful tool to study the biochemistry of down-regulation of an endogenous rice gene using a heterologous diamine-oxidase cDNA" @default.
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• W2019753453 doi "https://doi.org/10.1016/s0981-9428(00)01182-7" @default.
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