Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019772560> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2019772560 endingPage "237" @default.
- W2019772560 startingPage "230" @default.
- W2019772560 abstract "Interleukin-13 receptor-targeted cytotoxin (IL13-PE38) is highly cytotoxic to human glioblastoma (GBM) cells. Although this molecule is being tested in a multicenter Phase III clinical trial (PRECISE Study) in patients with recurrent disease, the activity of IL13-PE38 when combined with radiation therapy has not been investigated.Cytotoxicity of IL13-PE38 to GBM cell lines was assessed by protein synthesis inhibition and clonogenic assays, and the growth of GBM cells receiving radiation was assessed by thymidine uptake assays. Expression of IL-13 receptor alpha2 (IL-13Ralpha2) messenger ribonucleic acid (mRNA) in GBM cells exposed to radiation was assessed by quantitative reverse transcriptase/polymerase chain reaction (RT-PCR) and IL-13R density by radiolabeled IL-13 binding assays.Prior irradiation of GBM cell lines followed by IL13-PE38 treatment did not enhance cytotoxicity; however, concomitant 5 Gy irradiation and IL13-PE38 treatment was highly cytotoxic to T98G, M059K, A172, and LN-229 cell lines as determined by cell viability assays. There was a statistically significant decrease in number of viable cells in IL13-PE38 and irradiated cells compared with irradiated cells alone (p < 0.05) or IL13-PE38 treated cells alone (p < 0.05). In contrast, U251, SN19, and U87MG cell lines did not show any combined effect. These results were confirmed by clonogenic assays. Although three GBM cell lines-U251, SN19, and A172-showed 2.8- to 13.9-fold upregulation of IL-13Ralpha2 mRNA expression at 6-24 h after exposure to 5 Gy radiation, specific binding of radiolabeled IL-13 to these cell lines did not improve.Our results suggest that concomitant radiation therapy and IL13-PE38 treatment may be beneficial for the treatment of patients with GBM. This strategy may be worth exploring in animal models of human glioma." @default.
- W2019772560 created "2016-06-24" @default.
- W2019772560 creator A5037731704 @default.
- W2019772560 creator A5067972591 @default.
- W2019772560 creator A5082991756 @default.
- W2019772560 creator A5085028455 @default.
- W2019772560 date "2005-09-01" @default.
- W2019772560 modified "2023-09-22" @default.
- W2019772560 title "Combined effects of radiation and interleukin-13 receptor-targeted cytotoxin on glioblastoma cell lines" @default.
- W2019772560 cites W116096765 @default.
- W2019772560 cites W1541033317 @default.
- W2019772560 cites W1975489275 @default.
- W2019772560 cites W1983852551 @default.
- W2019772560 cites W1989750667 @default.
- W2019772560 cites W1990408233 @default.
- W2019772560 cites W2014707230 @default.
- W2019772560 cites W2023668782 @default.
- W2019772560 cites W2056011525 @default.
- W2019772560 cites W2066475464 @default.
- W2019772560 cites W2069743461 @default.
- W2019772560 cites W2077709559 @default.
- W2019772560 cites W2089301184 @default.
- W2019772560 cites W2094163515 @default.
- W2019772560 cites W2109839172 @default.
- W2019772560 cites W2135062838 @default.
- W2019772560 cites W2149955599 @default.
- W2019772560 cites W2169177355 @default.
- W2019772560 cites W21779961 @default.
- W2019772560 cites W4313333805 @default.
- W2019772560 doi "https://doi.org/10.1016/j.ijrobp.2005.05.017" @default.
- W2019772560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16111594" @default.
- W2019772560 hasPublicationYear "2005" @default.
- W2019772560 type Work @default.
- W2019772560 sameAs 2019772560 @default.
- W2019772560 citedByCount "11" @default.
- W2019772560 countsByYear W20197725602015 @default.
- W2019772560 countsByYear W20197725602017 @default.
- W2019772560 countsByYear W20197725602019 @default.
- W2019772560 countsByYear W20197725602020 @default.
- W2019772560 countsByYear W20197725602022 @default.
- W2019772560 countsByYear W20197725602023 @default.
- W2019772560 crossrefType "journal-article" @default.
- W2019772560 hasAuthorship W2019772560A5037731704 @default.
- W2019772560 hasAuthorship W2019772560A5067972591 @default.
- W2019772560 hasAuthorship W2019772560A5082991756 @default.
- W2019772560 hasAuthorship W2019772560A5085028455 @default.
- W2019772560 hasConcept C109316439 @default.
- W2019772560 hasConcept C117262875 @default.
- W2019772560 hasConcept C126322002 @default.
- W2019772560 hasConcept C153911025 @default.
- W2019772560 hasConcept C154317977 @default.
- W2019772560 hasConcept C170493617 @default.
- W2019772560 hasConcept C202751555 @default.
- W2019772560 hasConcept C2780783641 @default.
- W2019772560 hasConcept C502942594 @default.
- W2019772560 hasConcept C54355233 @default.
- W2019772560 hasConcept C55493867 @default.
- W2019772560 hasConcept C71924100 @default.
- W2019772560 hasConcept C81885089 @default.
- W2019772560 hasConcept C86803240 @default.
- W2019772560 hasConceptScore W2019772560C109316439 @default.
- W2019772560 hasConceptScore W2019772560C117262875 @default.
- W2019772560 hasConceptScore W2019772560C126322002 @default.
- W2019772560 hasConceptScore W2019772560C153911025 @default.
- W2019772560 hasConceptScore W2019772560C154317977 @default.
- W2019772560 hasConceptScore W2019772560C170493617 @default.
- W2019772560 hasConceptScore W2019772560C202751555 @default.
- W2019772560 hasConceptScore W2019772560C2780783641 @default.
- W2019772560 hasConceptScore W2019772560C502942594 @default.
- W2019772560 hasConceptScore W2019772560C54355233 @default.
- W2019772560 hasConceptScore W2019772560C55493867 @default.
- W2019772560 hasConceptScore W2019772560C71924100 @default.
- W2019772560 hasConceptScore W2019772560C81885089 @default.
- W2019772560 hasConceptScore W2019772560C86803240 @default.
- W2019772560 hasIssue "1" @default.
- W2019772560 hasLocation W20197725601 @default.
- W2019772560 hasLocation W20197725602 @default.
- W2019772560 hasOpenAccess W2019772560 @default.
- W2019772560 hasPrimaryLocation W20197725601 @default.
- W2019772560 hasRelatedWork W1970133830 @default.
- W2019772560 hasRelatedWork W1974029411 @default.
- W2019772560 hasRelatedWork W2058463700 @default.
- W2019772560 hasRelatedWork W2155481772 @default.
- W2019772560 hasRelatedWork W2357370706 @default.
- W2019772560 hasRelatedWork W2394757359 @default.
- W2019772560 hasRelatedWork W3102337599 @default.
- W2019772560 hasRelatedWork W4206956544 @default.
- W2019772560 hasRelatedWork W4249030396 @default.
- W2019772560 hasRelatedWork W83647411 @default.
- W2019772560 hasVolume "63" @default.
- W2019772560 isParatext "false" @default.
- W2019772560 isRetracted "false" @default.
- W2019772560 magId "2019772560" @default.
- W2019772560 workType "article" @default.