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• W2019791808 endingPage "406" @default.
• W2019791808 startingPage "397" @default.
• W2019791808 abstract "Epoxy resins provide optimal tissue morphology at both the light and the electron microscopic level and therefore enable correlative studies on semithin and thin sections from the same tissue block. Here we report on an approach to retain these advantages for immunolabeling studies by adapting and combining well-known techniques, i.e., surface etching with sodium ethoxide and heat-mediated antigen retrieval. We propose a simple procedure for immunostaining semithin and thin epoxy resin sections. To check its applicability, well characterized, commercially available antibodies (against E-cadherin, alpha-catenin, and beta-catenin) were used on sections of human small intestine. By light microscopy, the immunostaining efficiency was compared on cryo-, paraffin, and epoxy semithin sections processed in parallel. The most detailed results were obtained on semithin sections, where the labeling precisely delineated the lateral plasma membrane of the enterocytes. At the electron microscopic level the procedure did not damage the structures and allowed an efficient, reproducible immunogold labeling extending homogeneously over exceptionally wide tissue areas. The three antibodies specifically labeled the zonula adherens of the junctional complex between epithelial cells and, in agreement with light microscopic observations, the lateral plasma membrane." @default.
• W2019791808 created "2016-06-24" @default.
• W2019791808 creator A5015629464 @default.
• W2019791808 creator A5046752102 @default.
• W2019791808 creator A5053021061 @default.
• W2019791808 date "2001-03-01" @default.
• W2019791808 modified "2023-10-16" @default.
• W2019791808 title "Re-evaluation of Epoxy Resin Sections for Light and Electron Microscopic Immunostaining" @default.
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• W2019791808 doi "https://doi.org/10.1177/002215540104900313" @default.
• W2019791808 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11181742" @default.
• W2019791808 hasPublicationYear "2001" @default.
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