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• W2019810108 abstract "In this paper, we developed a simple and effective on-line focusing technique combining dynamic pH junction and sweeping by capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection. Dynamic pH junction-sweeping is defined when the sample has a different buffer pH (dynamic pH junction condition) and is devoid of micelles (sweeping condition) relative to the background electrolyte (BGE). This hyphenated focusing mode was applied to the sensitive and selective focusing of four dipeptides: Tyr-Phe, Tyr-Leu, Trp-Gly, and Ala-Gln. Picomolar detectability of these dipeptides by CE-LIF detection was demonstrated through effective focusing of large sample volumes (up to 39% capillary length) using the dual pH junction-sweeping focusing mode. 25 mmol L(-1) sodium dihydrogen phosphate, pH 2.5 was used as the sample matrix, and 100 mmol L(-1) borate, 21 mmol L(-1) sodium dodecylsulfate (SDS), 16 mmol L(-1) Brij35, pH 9.0 as the background solution (BGS). The concentration detection limits (S/N = 3) of the four dipeptides were in the range of 1.0-5.0 pmol L(-1). The developed method has been successfully used for the determination of dipeptides in human serum samples." @default.
• W2019810108 created "2016-06-24" @default.
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• W2019810108 creator A5038766133 @default.
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• W2019810108 date "2011-01-01" @default.
• W2019810108 modified "2023-10-07" @default.
• W2019810108 title "Dynamic pH junction–sweeping for on-line focusing of dipeptides in capillary electrophoresis with laser-induced fluorescence detection" @default.
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• W2019810108 doi "https://doi.org/10.1039/c0an00744g" @default.
• W2019810108 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21394345" @default.
• W2019810108 hasPublicationYear "2011" @default.
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