FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019889059> ?p ?o ?g. }

• W2019889059 endingPage "114" @default.
• W2019889059 startingPage "109" @default.
• W2019889059 abstract "We report a single, one-step PCR approach for detection and discrimination of Clonorchis sinensis and Opisthorchis viverrini in different life-stage forms (adults, metacercariae, and eggs) from fish intermediate hosts and from infected patients. Primers designed for species-specific PCR, amplifying portions of the mitochondrial (mt) genome, were also suitable for a multiplex PCR. The latter was a single, one-step reaction under high stringency conditions, using simultaneously 2 pairs of primers (1 pair for C. sinensis—product size 612 bp, and 1 pair for O. viverrini—product size 1357 bp). Assays using serially diluted templates demonstrated that as little as 0.78 ng of genomic DNA of either species could yield amplicons. Genomic DNA extracted from different life-stage forms including adult worms (of both species), eggs (of O. viverrini), eggs possibly of several trematode species (collected from patients infected with C. sinensis in Vietnam) and mixed metacercariae of common trematodes (collected from fishes in the C. sinensis endemic areas), yielded specific bands of the correct size and their identity was confirmed by sequence analysis. The multiplex PCR approach described here proved to be a species-specific, sensitive and fast tool for accurate diagnosis of clonorchiasis and/or opisthorchiasis, permitting the detection of their metacercariae in infected fishes or adult/eggs from patients in endemic areas." @default.
• W2019889059 created "2016-06-24" @default.
• W2019889059 creator A5015201498 @default.
• W2019889059 creator A5043460163 @default.
• W2019889059 creator A5054985463 @default.
• W2019889059 creator A5075583603 @default.
• W2019889059 creator A5089572924 @default.
• W2019889059 date "2006-02-01" @default.
• W2019889059 modified "2023-10-17" @default.
• W2019889059 title "Clonorchis sinensis and Opisthorchis viverrini: Development of a mitochondrial-based multiplex PCR for their identification and discrimination" @default.
• W2019889059 cites W1981811208 @default.
• W2019889059 cites W1999808461 @default.
• W2019889059 cites W2001978229 @default.
• W2019889059 cites W2003980807 @default.
• W2019889059 cites W2017034684 @default.
• W2019889059 cites W2030200185 @default.
• W2019889059 cites W2031995380 @default.
• W2019889059 cites W2039952752 @default.
• W2019889059 cites W2051275239 @default.
• W2019889059 cites W2062374917 @default.
• W2019889059 cites W2063016837 @default.
• W2019889059 cites W2063452779 @default.
• W2019889059 cites W2064938375 @default.
• W2019889059 cites W2080689337 @default.
• W2019889059 cites W2083827013 @default.
• W2019889059 cites W2106713576 @default.
• W2019889059 cites W2110906888 @default.
• W2019889059 cites W2116496596 @default.
• W2019889059 cites W2118720488 @default.
• W2019889059 cites W2127408642 @default.
• W2019889059 cites W2128671243 @default.
• W2019889059 cites W2151583953 @default.
• W2019889059 cites W2406884746 @default.
• W2019889059 doi "https://doi.org/10.1016/j.exppara.2005.09.012" @default.
• W2019889059 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16310774" @default.
• W2019889059 hasPublicationYear "2006" @default.
• W2019889059 type Work @default.
• W2019889059 sameAs 2019889059 @default.
• W2019889059 citedByCount "95" @default.
• W2019889059 countsByYear W20198890592012 @default.
• W2019889059 countsByYear W20198890592013 @default.
• W2019889059 countsByYear W20198890592014 @default.
• W2019889059 countsByYear W20198890592015 @default.
• W2019889059 countsByYear W20198890592016 @default.
• W2019889059 countsByYear W20198890592017 @default.
• W2019889059 countsByYear W20198890592018 @default.
• W2019889059 countsByYear W20198890592019 @default.
• W2019889059 countsByYear W20198890592020 @default.
• W2019889059 countsByYear W20198890592021 @default.
• W2019889059 countsByYear W20198890592022 @default.
• W2019889059 crossrefType "journal-article" @default.
• W2019889059 hasAuthorship W2019889059A5015201498 @default.
• W2019889059 hasAuthorship W2019889059A5043460163 @default.
• W2019889059 hasAuthorship W2019889059A5054985463 @default.
• W2019889059 hasAuthorship W2019889059A5075583603 @default.
• W2019889059 hasAuthorship W2019889059A5089572924 @default.
• W2019889059 hasConcept C104317684 @default.
• W2019889059 hasConcept C13590703 @default.
• W2019889059 hasConcept C142724271 @default.
• W2019889059 hasConcept C153911025 @default.
• W2019889059 hasConcept C159047783 @default.
• W2019889059 hasConcept C165901193 @default.
• W2019889059 hasConcept C17757408 @default.
• W2019889059 hasConcept C24586158 @default.
• W2019889059 hasConcept C2775879618 @default.
• W2019889059 hasConcept C2778040090 @default.
• W2019889059 hasConcept C2780238400 @default.
• W2019889059 hasConcept C2780431383 @default.
• W2019889059 hasConcept C2780838300 @default.
• W2019889059 hasConcept C49105822 @default.
• W2019889059 hasConcept C54355233 @default.
• W2019889059 hasConcept C71924100 @default.
• W2019889059 hasConcept C8185291 @default.
• W2019889059 hasConcept C86803240 @default.
• W2019889059 hasConcept C90583042 @default.
• W2019889059 hasConcept C90856448 @default.
• W2019889059 hasConceptScore W2019889059C104317684 @default.
• W2019889059 hasConceptScore W2019889059C13590703 @default.
• W2019889059 hasConceptScore W2019889059C142724271 @default.
• W2019889059 hasConceptScore W2019889059C153911025 @default.
• W2019889059 hasConceptScore W2019889059C159047783 @default.
• W2019889059 hasConceptScore W2019889059C165901193 @default.
• W2019889059 hasConceptScore W2019889059C17757408 @default.
• W2019889059 hasConceptScore W2019889059C24586158 @default.
• W2019889059 hasConceptScore W2019889059C2775879618 @default.
• W2019889059 hasConceptScore W2019889059C2778040090 @default.
• W2019889059 hasConceptScore W2019889059C2780238400 @default.
• W2019889059 hasConceptScore W2019889059C2780431383 @default.
• W2019889059 hasConceptScore W2019889059C2780838300 @default.
• W2019889059 hasConceptScore W2019889059C49105822 @default.
• W2019889059 hasConceptScore W2019889059C54355233 @default.
• W2019889059 hasConceptScore W2019889059C71924100 @default.
• W2019889059 hasConceptScore W2019889059C8185291 @default.
• W2019889059 hasConceptScore W2019889059C86803240 @default.
• W2019889059 hasConceptScore W2019889059C90583042 @default.
• W2019889059 hasConceptScore W2019889059C90856448 @default.
• W2019889059 hasIssue "2" @default.
• W2019889059 hasLocation W20198890591 @default.

• next