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- W2019901113 abstract "Colicin E3 is a bacteriocin which inhibits protein synthesis by cleaving about 50 nucleotides from the 3’-terminus of 16 S rRNA [1,2]. The role of 16 S rRNA in protein synthesis has been studied by using colicin E3 or cloacin DF13 [3-IO]. If colicin E3 similarly inactivates mammalian ribosomes, colicin E3 becomes a good tool to study the structure and function of mammalian ribosomes. In vitro inactivation of mammalian ribosomes by colicin E3 was first reported [ 111, and this may be the only work that observed the effect of colicin E3 on mammalian ribosomes. The rabbit reticulocyte lysate system has been widely used to study protein synthesis, and much information has accumulated. Therefore, we studied the effect of colicin E3 on our lysate system. This paper reports inhibition of rabbit globin synthesis in the rabbit reticulocyte lysate system. Globin synthesis exponentially decreased with the time of preincubation of the lysate with colicin E3. Both (Yand @globin synthesis were inhibited similarly. By preincubation of the lysate with colicin E3, the formation of the 80 S initiation complex was inhibited, but not that of the 40 S initiation complex." @default.
- W2019901113 created "2016-06-24" @default.
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- W2019901113 date "1978-05-01" @default.
- W2019901113 modified "2023-09-24" @default.
- W2019901113 title "Colicin E3 inhibits rabbit globin synthesis" @default.
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- W2019901113 doi "https://doi.org/10.1016/0014-5793(78)80536-5" @default.
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