Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019904364> ?p ?o ?g. }
- W2019904364 abstract "We have carried out a screen of 622 deletion strains generated during the EUROFAN B0 project to identify non-essential genes related to the mannosylphosphate content of the cell wall. By examining the affinity of the deletants for the cationic dye alcian blue and the ion exchanger QAE-Sephadex, we have selected 50 strains. On the basis on their reactivity (blue colour intensity) in the alcian blue assay, mutants with a lower phosphate content than wild-type cells were then arranged in groups defined by previously characterized mutants, as follows: group I (mnn6), group II (between mnn6 and mnn9) and group III (mnn9). Similarly, strains that behaved like mnn1 (i.e. a blue colour deeper than wild-type) were included in group VI. To confirm the association between the phenotype and a specific mutation, strains were complemented with clones or subjected to tetrad analysis. Selected strains were further tested for extracellular invertase and exoglucanase. Within groups I, II and III, we found some genes known to be involved in oligosaccharide biosynthesis (ALG9, ALG12, HOC1), secretion (BRE5, COD4/COG5, VPS53), transcription (YOL072w/THP1, ELP2, STB1, SNF11), cell polarity (SEP7, RDG1), mitochondrial function (YFH1), cell metabolism, as well as orphan genes. Within group VI, we found genes involved in environmentally regulated transduction pathways (PAL2 and RIM20) as well as others with miscellaneous or unknown functions. We conclude that mannosylphosphorylation is severely impaired in some deletants deficient in specific glycosylation/secretion processes, but many other different pathways may also modulate the amount of mannosylphosphate in the cell wall." @default.
- W2019904364 created "2016-06-24" @default.
- W2019904364 creator A5033190295 @default.
- W2019904364 creator A5036027102 @default.
- W2019904364 creator A5058020739 @default.
- W2019904364 creator A5085307609 @default.
- W2019904364 date "2003-01-01" @default.
- W2019904364 modified "2023-10-16" @default.
- W2019904364 title "Screening for new yeast mutants affected in mannosylphosphorylation of cell wall mannoproteins" @default.
- W2019904364 cites W1505467882 @default.
- W2019904364 cites W1553589824 @default.
- W2019904364 cites W1658092509 @default.
- W2019904364 cites W1822930845 @default.
- W2019904364 cites W1927235811 @default.
- W2019904364 cites W1943761658 @default.
- W2019904364 cites W1964827643 @default.
- W2019904364 cites W1964997225 @default.
- W2019904364 cites W1965498254 @default.
- W2019904364 cites W1966150808 @default.
- W2019904364 cites W1967432418 @default.
- W2019904364 cites W1968112541 @default.
- W2019904364 cites W1971895475 @default.
- W2019904364 cites W1975456619 @default.
- W2019904364 cites W1978966221 @default.
- W2019904364 cites W1983635742 @default.
- W2019904364 cites W1984924767 @default.
- W2019904364 cites W1993228561 @default.
- W2019904364 cites W1993613706 @default.
- W2019904364 cites W1997299080 @default.
- W2019904364 cites W1999196262 @default.
- W2019904364 cites W1999355784 @default.
- W2019904364 cites W2000192946 @default.
- W2019904364 cites W2001923796 @default.
- W2019904364 cites W2002341882 @default.
- W2019904364 cites W2003004599 @default.
- W2019904364 cites W2004344971 @default.
- W2019904364 cites W2004741118 @default.
- W2019904364 cites W2004828705 @default.
- W2019904364 cites W2007396399 @default.
- W2019904364 cites W2014656485 @default.
- W2019904364 cites W2015530645 @default.
- W2019904364 cites W2018529819 @default.
- W2019904364 cites W2018979952 @default.
- W2019904364 cites W2021662650 @default.
- W2019904364 cites W2026164783 @default.
- W2019904364 cites W2028234211 @default.
- W2019904364 cites W2034452896 @default.
- W2019904364 cites W2036277695 @default.
- W2019904364 cites W2043282701 @default.
- W2019904364 cites W2045717118 @default.
- W2019904364 cites W2046900167 @default.
- W2019904364 cites W2049094344 @default.
- W2019904364 cites W2049144892 @default.
- W2019904364 cites W2051178022 @default.
- W2019904364 cites W2055938272 @default.
- W2019904364 cites W2057580129 @default.
- W2019904364 cites W2058571066 @default.
- W2019904364 cites W2059356307 @default.
- W2019904364 cites W2060209297 @default.
- W2019904364 cites W2062983176 @default.
- W2019904364 cites W2064055793 @default.
- W2019904364 cites W2065230651 @default.
- W2019904364 cites W2069849662 @default.
- W2019904364 cites W2075506671 @default.
- W2019904364 cites W2077141078 @default.
- W2019904364 cites W2077580489 @default.
- W2019904364 cites W2078101367 @default.
- W2019904364 cites W2079815614 @default.
- W2019904364 cites W2097249829 @default.
- W2019904364 cites W2098098380 @default.
- W2019904364 cites W2098999612 @default.
- W2019904364 cites W2100057937 @default.
- W2019904364 cites W2101107183 @default.
- W2019904364 cites W2102285200 @default.
- W2019904364 cites W2102716917 @default.
- W2019904364 cites W2103111390 @default.
- W2019904364 cites W2105190386 @default.
- W2019904364 cites W2109449816 @default.
- W2019904364 cites W2111379154 @default.
- W2019904364 cites W2115685883 @default.
- W2019904364 cites W2118006330 @default.
- W2019904364 cites W2118758150 @default.
- W2019904364 cites W2119097563 @default.
- W2019904364 cites W2120959840 @default.
- W2019904364 cites W2123635521 @default.
- W2019904364 cites W2123738381 @default.
- W2019904364 cites W2127754721 @default.
- W2019904364 cites W2133201628 @default.
- W2019904364 cites W2133332070 @default.
- W2019904364 cites W2134626310 @default.
- W2019904364 cites W2137798826 @default.
- W2019904364 cites W2138326207 @default.
- W2019904364 cites W2142460132 @default.
- W2019904364 cites W2143617365 @default.
- W2019904364 cites W2144036800 @default.
- W2019904364 cites W2147300206 @default.
- W2019904364 cites W2147361648 @default.
- W2019904364 cites W2147456502 @default.
- W2019904364 cites W2150541674 @default.
- W2019904364 cites W2151585536 @default.