Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019915182> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2019915182 endingPage "187" @default.
- W2019915182 startingPage "183" @default.
- W2019915182 abstract "To improve the thermal stability and cellulose-binding capacity of Cryptococcus sp. S-2 lipase (CSLP), the cellulose-binding domain originates from Trichoderma reesei cellobiohydrolase I was engineered into C-terminal region of the CSLP (CSLP-CBD). The CSLP and CSLP-CBD were successfully expressed in the Pichia pastoris using the strong methanol inducible alcohol oxidase 1 (AOX1) promoter and the secretion signal sequence from Saccharomyces cerevisiae (α factor). The recombinant CSLP and CSLP-CBD were secreted into culture medium and estimated by SDS–PAGE to be 22 and 27 kDa, respectively. The fusion enzyme was stable at 80 °C and retained more than 80% of its activity after 120-min incubation at this temperature. Our results also found that the fusion of fungal exoglucanase cellulose-binding domain to CSLP is responsible for cellulose-binding capacity. This attribute should make it an attractive applicant for enzyme immobilization." @default.
- W2019915182 created "2016-06-24" @default.
- W2019915182 creator A5000349175 @default.
- W2019915182 creator A5018108394 @default.
- W2019915182 creator A5025226768 @default.
- W2019915182 date "2012-03-01" @default.
- W2019915182 modified "2023-10-02" @default.
- W2019915182 title "Increases thermal stability and cellulose-binding capacity of Cryptococcus sp. S-2 lipase by fusion of cellulose binding domain derived from Trichoderma reesei" @default.
- W2019915182 cites W1952322446 @default.
- W2019915182 cites W1971720195 @default.
- W2019915182 cites W1973163826 @default.
- W2019915182 cites W1973767358 @default.
- W2019915182 cites W1991890196 @default.
- W2019915182 cites W2039548376 @default.
- W2019915182 cites W2052049981 @default.
- W2019915182 cites W2061208926 @default.
- W2019915182 cites W2062767843 @default.
- W2019915182 cites W2068305910 @default.
- W2019915182 cites W2069360880 @default.
- W2019915182 cites W2076184881 @default.
- W2019915182 cites W2100837269 @default.
- W2019915182 cites W2144423272 @default.
- W2019915182 cites W2147665045 @default.
- W2019915182 cites W2166103682 @default.
- W2019915182 cites W2339567916 @default.
- W2019915182 cites W2412152036 @default.
- W2019915182 cites W4293247451 @default.
- W2019915182 doi "https://doi.org/10.1016/j.bbrc.2012.02.139" @default.
- W2019915182 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22405828" @default.
- W2019915182 hasPublicationYear "2012" @default.
- W2019915182 type Work @default.
- W2019915182 sameAs 2019915182 @default.
- W2019915182 citedByCount "17" @default.
- W2019915182 countsByYear W20199151822013 @default.
- W2019915182 countsByYear W20199151822014 @default.
- W2019915182 countsByYear W20199151822015 @default.
- W2019915182 countsByYear W20199151822016 @default.
- W2019915182 countsByYear W20199151822017 @default.
- W2019915182 countsByYear W20199151822019 @default.
- W2019915182 countsByYear W20199151822021 @default.
- W2019915182 countsByYear W20199151822022 @default.
- W2019915182 crossrefType "journal-article" @default.
- W2019915182 hasAuthorship W2019915182A5000349175 @default.
- W2019915182 hasAuthorship W2019915182A5018108394 @default.
- W2019915182 hasAuthorship W2019915182A5025226768 @default.
- W2019915182 hasConcept C104317684 @default.
- W2019915182 hasConcept C141603559 @default.
- W2019915182 hasConcept C185592680 @default.
- W2019915182 hasConcept C2776125036 @default.
- W2019915182 hasConcept C2779251873 @default.
- W2019915182 hasConcept C2781372317 @default.
- W2019915182 hasConcept C40767141 @default.
- W2019915182 hasConcept C43617362 @default.
- W2019915182 hasConcept C55493867 @default.
- W2019915182 hasConceptScore W2019915182C104317684 @default.
- W2019915182 hasConceptScore W2019915182C141603559 @default.
- W2019915182 hasConceptScore W2019915182C185592680 @default.
- W2019915182 hasConceptScore W2019915182C2776125036 @default.
- W2019915182 hasConceptScore W2019915182C2779251873 @default.
- W2019915182 hasConceptScore W2019915182C2781372317 @default.
- W2019915182 hasConceptScore W2019915182C40767141 @default.
- W2019915182 hasConceptScore W2019915182C43617362 @default.
- W2019915182 hasConceptScore W2019915182C55493867 @default.
- W2019915182 hasIssue "1" @default.
- W2019915182 hasLocation W20199151821 @default.
- W2019915182 hasLocation W20199151822 @default.
- W2019915182 hasOpenAccess W2019915182 @default.
- W2019915182 hasPrimaryLocation W20199151821 @default.
- W2019915182 hasRelatedWork W1973580547 @default.
- W2019915182 hasRelatedWork W2005353501 @default.
- W2019915182 hasRelatedWork W2030236832 @default.
- W2019915182 hasRelatedWork W2054362110 @default.
- W2019915182 hasRelatedWork W2150544704 @default.
- W2019915182 hasRelatedWork W2200746345 @default.
- W2019915182 hasRelatedWork W2390151155 @default.
- W2019915182 hasRelatedWork W2971839875 @default.
- W2019915182 hasRelatedWork W346366706 @default.
- W2019915182 hasRelatedWork W4312618366 @default.
- W2019915182 hasVolume "420" @default.
- W2019915182 isParatext "false" @default.
- W2019915182 isRetracted "false" @default.
- W2019915182 magId "2019915182" @default.
- W2019915182 workType "article" @default.