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- W2019916556 abstract "A method for the determination of oxalate in urine is described. Oxalate is decarboxylated with oxalate decarboxylase (E.C. 4.1.1.2) in vials equipped with rubber septa. Carbon dioxide generated is sampled with a gas-tight syringe, injected into a methanation oven and detected with a flame ionization detector. A simple one-point kinetic method is suggested because enzyme inhibition from sulphate and phosphate may cause incomplete conversion of oxalate. The choice of pH for the method described is discussed in the light of the effect of pH on the Michaelis constant, on the maximum velocity and on inhibition constants. It was estimated that 50 μ mol l−1 oxalate can be determined with a precision of 10%." @default.
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- W2019916556 date "1983-01-01" @default.
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- W2019916556 title "Enzymatic determination of oxalate in urine by headspace sampling of carbon dioxide and flame ionization detection" @default.
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- W2019916556 doi "https://doi.org/10.1016/s0003-2670(00)84902-8" @default.
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