Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019919212> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2019919212 endingPage "1463" @default.
- W2019919212 startingPage "1453" @default.
- W2019919212 abstract "During cell line development for an IgG1 antibody candidate (mAb1), a C-terminal extension was identified in 2 product candidate clones expressed in CHO-K1 cell line. The extension was initially observed as the presence of anomalous new peaks in these clones after analysis by cation exchange chromatography (CEX-HPLC) and reduced capillary electrophoresis (rCE-SDS). Reduced mass analysis of these CHO-K1 clones revealed that a larger than expected mass was present on a sub-population of the heavy chain species, which could not be explained by any known chemical or post-translational modifications. It was suspected that this additional mass on the heavy chain was due to the presence of an additional amino acid sequence. To identify the suspected additional sequence, de novo sequencing in combination with proteomic searching was performed against translated DNA vectors for the heavy chain and light chain. Peptides unique to the clones containing the extension were identified matching short sequences (corresponding to 9 and 35 amino acids, respectively) from 2 non-coding sections of the light chain vector construct. After investigation, this extension was observed to be due to the re-arrangement of the DNA construct, with the addition of amino acids derived from the light chain vector non-translated sequence to the C-terminus of the heavy chain. This observation showed the power of proteomic mass spectrometric techniques to identify an unexpected antibody sequence variant using de novo sequencing combined with database searching, and allowed for rapid identification of the root cause for new peaks in the cation exchange and rCE-SDS assays." @default.
- W2019919212 created "2016-06-24" @default.
- W2019919212 creator A5047321575 @default.
- W2019919212 creator A5062806679 @default.
- W2019919212 creator A5079589390 @default.
- W2019919212 creator A5082807745 @default.
- W2019919212 date "2014-11-02" @default.
- W2019919212 modified "2023-10-01" @default.
- W2019919212 title "Rapid identification of an antibody DNA construct rearrangement sequence variant by mass spectrometry" @default.
- W2019919212 cites W1966867878 @default.
- W2019919212 cites W1979741421 @default.
- W2019919212 cites W1988701639 @default.
- W2019919212 cites W2059117829 @default.
- W2019919212 cites W2071922186 @default.
- W2019919212 cites W2076667355 @default.
- W2019919212 cites W2087470177 @default.
- W2019919212 cites W2091360947 @default.
- W2019919212 cites W2093052021 @default.
- W2019919212 cites W2098410748 @default.
- W2019919212 cites W2168473498 @default.
- W2019919212 cites W2318596598 @default.
- W2019919212 doi "https://doi.org/10.4161/mabs.36222" @default.
- W2019919212 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4622602" @default.
- W2019919212 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25484040" @default.
- W2019919212 hasPublicationYear "2014" @default.
- W2019919212 type Work @default.
- W2019919212 sameAs 2019919212 @default.
- W2019919212 citedByCount "19" @default.
- W2019919212 countsByYear W20199192122015 @default.
- W2019919212 countsByYear W20199192122016 @default.
- W2019919212 countsByYear W20199192122017 @default.
- W2019919212 countsByYear W20199192122018 @default.
- W2019919212 countsByYear W20199192122019 @default.
- W2019919212 countsByYear W20199192122021 @default.
- W2019919212 countsByYear W20199192122022 @default.
- W2019919212 countsByYear W20199192122023 @default.
- W2019919212 crossrefType "journal-article" @default.
- W2019919212 hasAuthorship W2019919212A5047321575 @default.
- W2019919212 hasAuthorship W2019919212A5062806679 @default.
- W2019919212 hasAuthorship W2019919212A5079589390 @default.
- W2019919212 hasAuthorship W2019919212A5082807745 @default.
- W2019919212 hasBestOaLocation W20199192121 @default.
- W2019919212 hasConcept C104317684 @default.
- W2019919212 hasConcept C153911025 @default.
- W2019919212 hasConcept C159654299 @default.
- W2019919212 hasConcept C162356407 @default.
- W2019919212 hasConcept C167625842 @default.
- W2019919212 hasConcept C185592680 @default.
- W2019919212 hasConcept C31827203 @default.
- W2019919212 hasConcept C36394416 @default.
- W2019919212 hasConcept C43617362 @default.
- W2019919212 hasConcept C515207424 @default.
- W2019919212 hasConcept C51679486 @default.
- W2019919212 hasConcept C54355233 @default.
- W2019919212 hasConcept C552990157 @default.
- W2019919212 hasConcept C55493867 @default.
- W2019919212 hasConcept C61053724 @default.
- W2019919212 hasConcept C70721500 @default.
- W2019919212 hasConcept C86803240 @default.
- W2019919212 hasConceptScore W2019919212C104317684 @default.
- W2019919212 hasConceptScore W2019919212C153911025 @default.
- W2019919212 hasConceptScore W2019919212C159654299 @default.
- W2019919212 hasConceptScore W2019919212C162356407 @default.
- W2019919212 hasConceptScore W2019919212C167625842 @default.
- W2019919212 hasConceptScore W2019919212C185592680 @default.
- W2019919212 hasConceptScore W2019919212C31827203 @default.
- W2019919212 hasConceptScore W2019919212C36394416 @default.
- W2019919212 hasConceptScore W2019919212C43617362 @default.
- W2019919212 hasConceptScore W2019919212C515207424 @default.
- W2019919212 hasConceptScore W2019919212C51679486 @default.
- W2019919212 hasConceptScore W2019919212C54355233 @default.
- W2019919212 hasConceptScore W2019919212C552990157 @default.
- W2019919212 hasConceptScore W2019919212C55493867 @default.
- W2019919212 hasConceptScore W2019919212C61053724 @default.
- W2019919212 hasConceptScore W2019919212C70721500 @default.
- W2019919212 hasConceptScore W2019919212C86803240 @default.
- W2019919212 hasIssue "6" @default.
- W2019919212 hasLocation W20199192121 @default.
- W2019919212 hasLocation W20199192122 @default.
- W2019919212 hasLocation W20199192123 @default.
- W2019919212 hasLocation W20199192124 @default.
- W2019919212 hasOpenAccess W2019919212 @default.
- W2019919212 hasPrimaryLocation W20199192121 @default.
- W2019919212 hasRelatedWork W1501958583 @default.
- W2019919212 hasRelatedWork W2007396871 @default.
- W2019919212 hasRelatedWork W2011135376 @default.
- W2019919212 hasRelatedWork W2012595611 @default.
- W2019919212 hasRelatedWork W2052807334 @default.
- W2019919212 hasRelatedWork W2073375101 @default.
- W2019919212 hasRelatedWork W2171305187 @default.
- W2019919212 hasRelatedWork W4248558637 @default.
- W2019919212 hasRelatedWork W4250780869 @default.
- W2019919212 hasRelatedWork W56988916 @default.
- W2019919212 hasVolume "6" @default.
- W2019919212 isParatext "false" @default.
- W2019919212 isRetracted "false" @default.
- W2019919212 magId "2019919212" @default.
- W2019919212 workType "article" @default.