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• W2019922740 abstract "Analyses of cleavage ends of DNA fragments in apoptotic rat thymocytes induced by γ-ray irradiation or by treatment with dexamethasone revealed that in both cases the fragments produced had 3′-hydroxyl (OH) and 5′-phosphoryl (P) ends of DNA chains. Rat thymocyte nuclei contained at least three endonuclease activities (deoxyribonucleases α, β and γ) that were able to cleave chromatin to mononucleosomal and oligonucleosomal fragments. The nuclei of apoptotic rat thymocytes induced by γ-ray irradiation or dexamethasone retained considerable deoxyribonuclease γ activity, but not α or β deoxyribonuclease activity. During the induction of apoptosis, treatment with cycloheximide, which suppressed apoptosis, resulted in marked decreases of deoxyribonucleases α and β activities. After release of cycloheximide inhibition, DNA fragmentation associated with apoptosis occurred in the cycloheximide-treated thymocyte nuclei, in which deoxyribonuclease γ activity was only observed. The purified deoxyribonucleases α and β were divalent cation-independent acidic endonucleases, which were separated on a CM5PW column by HPLC. The molecular masses of deoxyribonucleases α and β were 28 kDa and 30 kDa, respectively, as determined by TSK G-2000SW gel-filtration HPLC, and both were 32 kDa in molecular mass as determined by SDS/PAGE. In contrast, deoxyribonuclease γ, a neutral endonuclease, required both Ca2+ and Mg2+ for full activity and was inhibited by Zn2+. The molecular mass of deoxyribonuclease γ was 31 kDa and 33 kDa when measured by gel filtration and SDS/PAGE, respectively. Under these optimal conditions, deoxyribonuclease γ was shown to produce 3′-OH/5′-P ends of nucleosomal DNA fragments, while deoxyribonucleases α and β both formed DNA fragments with 3′-P/5′-OH ends. The ends formed by cleavage with deoxyribonuclease γ were the same as those produced in apoptotic rat thymocytes. On the basis of these results, it seems likely that deoxyribonuclease γ is responsible for internucleosomal cleavage of chromatin during thymic apoptosis." @default.
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• W2019922740 date "2008-06-28" @default.
• W2019922740 modified "2023-10-17" @default.
• W2019922740 title "Identification of an Endonuclease Responsible for Apoptosis in Rat Thymocytes" @default.
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• W2019922740 doi "https://doi.org/10.1111/j.1432-1033.1994.00t23.x" @default.
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