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- W2019924261 abstract "Polyspermy was assayed by counting sperm pronuclei in orcein-stained eggs of Crassostrea gigas with ordinary light microscopy. When oocytes were inseminated within 15 min of their mechanical removal from the ovary, they became highly polyspermic, for example, 54% polyspermy with external spermoocyte ratios as low as 30. When they were incubated in seawater prior to insemination, susceptibility to polyspermy decreased with time, becoming quite low after 1 h (≤7% polyspermy with spermoocyte ratios up to 1000). Control experiments with fresh sperm showed the decline was due to changes in oocytes, not sperm. Spawned oocytes, obtained from females stimulated with dead sperm, were considerably more resistant to polyspermy than were oocytes mechanically removed from the ovary. Nevertheless, in three of the four lots tested, susceptibility to polyspermy also declined with time; oocytes inseminated immediately after they were spawned were more polyspermic than oocytes inseminated after 1–1.5 h in seawater. Percentages of fertilized eggs did not decline in ovarian and spawned oocytes during 1.5 h. Analysis of the data with the Poisson distribution supports the conclusion that a polyspermy block was weak or absent in recently obtained ovarian oocytes, and stronger in spawned oocytes and in ovarian oocytes incubated 1–1.5 h before insemination. There was not a good correlation between resistance to polyspermy and occurrence of spontaneous germinal vesicle breakdown prior to insemination. With both spawned and ovarian oocytes, there was a close correlation between percentage of polyspermic eggs and percentage of eggs with abnormal first cleavage (r=0.989), and between percentage of monospermic eggs and percentage of normal larvae (r=0.993). These correlations were observed whether oocytes were inseminated immediately or after incubation in seawater. Avoiding polyspermy is therefore an important prerequisite for obtaining high yields of normal larvae." @default.
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- W2019924261 date "1988-10-01" @default.
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- W2019924261 title "Avoiding polyspermy in oyster (Crassostrea gigas)" @default.
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- W2019924261 doi "https://doi.org/10.1016/0044-8486(88)90063-4" @default.
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