FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019926733> ?p ?o ?g. }

• W2019926733 endingPage "601" @default.
• W2019926733 startingPage "588" @default.
• W2019926733 abstract "Tn4451 is the paradigm element of a family of mobilizable chloramphenicol resistance transposons from Clostridium perfringens and Clostridium difficile. The unique feature of these 6.3 kb elements is that their excision to form a circular molecule is mediated by TnpX, a member of the large resolvase family of site-specific recombinases. By optimizing the transposition assay system in Escherichia coli, we showed that Tn4453a from C. difficile transposed at a higher frequency than the C. perfringens element, Tn4451, and that transposition of both Tn4451 and Tn4453a was significantly enhanced by the provision of a multicopy tnpX gene in trans. The complete nucleotide sequence of Tn4453a was determined, but its comparison with Tn4451 did not reveal why it transposed at a higher frequency. Using experiments involving a chromosomal derivative of Tn4453a, we have confirmed that the circular form is the transposition intermediate. As the tnpX gene is located very close to one end of these elements, primer extension analysis was used to determine the transcription start point. The results showed that the formation of the circular intermediate creates a strong tnpX promoter, which consists of a -10 box originally located at the left end of the transposon and a -35 box originally located at the right end. The data provide strong evidence that transcription of tnpX is likely to occur from the non-replicating circular intermediate, which would facilitate the subsequent insertion of the transient circular molecule. It is postulated that, when the transposon is in an integrated state, transcription of tnpX would depend on the presence of an appropriately spaced -35 sequence in the DNA flanking the insertion site or the presence of an alternative upstream promoter." @default.
• W2019926733 created "2016-06-24" @default.
• W2019926733 creator A5053064309 @default.
• W2019926733 creator A5081312384 @default.
• W2019926733 date "2000-11-01" @default.
• W2019926733 modified "2023-10-16" @default.
• W2019926733 title "Transposition of Tn4451 and Tn4453 involves a circular intermediate that forms a promoter for the large resolvase, TnpX" @default.
• W2019926733 cites W1653095775 @default.
• W2019926733 cites W1706293004 @default.
• W2019926733 cites W1765098394 @default.
• W2019926733 cites W1970469196 @default.
• W2019926733 cites W1971718161 @default.
• W2019926733 cites W1985543936 @default.
• W2019926733 cites W2013407009 @default.
• W2019926733 cites W2024163054 @default.
• W2019926733 cites W2025776320 @default.
• W2019926733 cites W2026248887 @default.
• W2019926733 cites W2031322963 @default.
• W2019926733 cites W2044688143 @default.
• W2019926733 cites W2050499271 @default.
• W2019926733 cites W2050537059 @default.
• W2019926733 cites W2084671891 @default.
• W2019926733 cites W2085290399 @default.
• W2019926733 cites W2090476579 @default.
• W2019926733 cites W2091590623 @default.
• W2019926733 cites W2093362279 @default.
• W2019926733 cites W2101339780 @default.
• W2019926733 cites W2107652872 @default.
• W2019926733 cites W2115704435 @default.
• W2019926733 cites W2123747707 @default.
• W2019926733 cites W2124111522 @default.
• W2019926733 cites W2127097525 @default.
• W2019926733 cites W2128435504 @default.
• W2019926733 cites W2130436333 @default.
• W2019926733 cites W2131964745 @default.
• W2019926733 cites W2134812217 @default.
• W2019926733 cites W2138537796 @default.
• W2019926733 cites W2161524167 @default.
• W2019926733 cites W2606310517 @default.
• W2019926733 cites W4236254039 @default.
• W2019926733 cites W66369755 @default.
• W2019926733 doi "https://doi.org/10.1046/j.1365-2958.2000.02154.x" @default.
• W2019926733 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11069682" @default.
• W2019926733 hasPublicationYear "2000" @default.
• W2019926733 type Work @default.
• W2019926733 sameAs 2019926733 @default.
• W2019926733 citedByCount "57" @default.
• W2019926733 countsByYear W20199267332013 @default.
• W2019926733 countsByYear W20199267332014 @default.
• W2019926733 countsByYear W20199267332015 @default.
• W2019926733 countsByYear W20199267332016 @default.
• W2019926733 countsByYear W20199267332017 @default.
• W2019926733 countsByYear W20199267332018 @default.
• W2019926733 countsByYear W20199267332019 @default.
• W2019926733 countsByYear W20199267332020 @default.
• W2019926733 countsByYear W20199267332021 @default.
• W2019926733 countsByYear W20199267332022 @default.
• W2019926733 countsByYear W20199267332023 @default.
• W2019926733 crossrefType "journal-article" @default.
• W2019926733 hasAuthorship W2019926733A5053064309 @default.
• W2019926733 hasAuthorship W2019926733A5081312384 @default.
• W2019926733 hasBestOaLocation W20199267331 @default.
• W2019926733 hasConcept C102414288 @default.
• W2019926733 hasConcept C104317684 @default.
• W2019926733 hasConcept C105793898 @default.
• W2019926733 hasConcept C12455157 @default.
• W2019926733 hasConcept C138885662 @default.
• W2019926733 hasConcept C141231307 @default.
• W2019926733 hasConcept C143065580 @default.
• W2019926733 hasConcept C153911025 @default.
• W2019926733 hasConcept C156695909 @default.
• W2019926733 hasConcept C172510086 @default.
• W2019926733 hasConcept C179926584 @default.
• W2019926733 hasConcept C41895202 @default.
• W2019926733 hasConcept C4918238 @default.
• W2019926733 hasConcept C51603236 @default.
• W2019926733 hasConcept C54355233 @default.
• W2019926733 hasConcept C86803240 @default.
• W2019926733 hasConceptScore W2019926733C102414288 @default.
• W2019926733 hasConceptScore W2019926733C104317684 @default.
• W2019926733 hasConceptScore W2019926733C105793898 @default.
• W2019926733 hasConceptScore W2019926733C12455157 @default.
• W2019926733 hasConceptScore W2019926733C138885662 @default.
• W2019926733 hasConceptScore W2019926733C141231307 @default.
• W2019926733 hasConceptScore W2019926733C143065580 @default.
• W2019926733 hasConceptScore W2019926733C153911025 @default.
• W2019926733 hasConceptScore W2019926733C156695909 @default.
• W2019926733 hasConceptScore W2019926733C172510086 @default.
• W2019926733 hasConceptScore W2019926733C179926584 @default.
• W2019926733 hasConceptScore W2019926733C41895202 @default.
• W2019926733 hasConceptScore W2019926733C4918238 @default.
• W2019926733 hasConceptScore W2019926733C51603236 @default.
• W2019926733 hasConceptScore W2019926733C54355233 @default.
• W2019926733 hasConceptScore W2019926733C86803240 @default.
• W2019926733 hasIssue "3" @default.
• W2019926733 hasLocation W20199267331 @default.
• W2019926733 hasLocation W20199267332 @default.
• W2019926733 hasOpenAccess W2019926733 @default.

• next