FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019927307> ?p ?o ?g. }

• W2019927307 endingPage "263" @default.
• W2019927307 startingPage "255" @default.
• W2019927307 abstract "Multivalency is a recognized means to increase the functional affinity of single-chain antibody fragments (scFvs) for optimizing tumor uptake at radioimmunotargeting. A unique divalent tandem single-chain Fv antibody [sc(Fv)2], based on the variable regions of the monoclonal antibody (MAb) H7, has now been generated. The antibody differs from other dimeric single-chain constructs in that a linker sequence (L) is introduced between the repeats of VL and VH domains (VL-L-VH-L-VL-L-VH). This construct was expressed as a His-tagged TrxA fusion protein in the Escherichia coli strain Origami B. Following cleavage with AcTev protease, the antibody was obtained in soluble and active form in E. coli and could be purified by Ni-NTA and cation-exchange chromatography. Purity and immunochemical properties were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), enzyme-linked immunosorbent assay (ELISA), Western blot, and Biacore analyses. The [sc(Fv)2] displayed proper stability and could be purified to homogeneity. This antibody also maintained immunoreactivity at 42°C with only slight decrease at 52°C. The high affinity displayed by the original antibody H7, 6.7 × 109 M–1, was only slightly decreased to 1.2 × 109 M–1 as determined by Biacore. The generation of such a divalent single-chain Fv with a molecular weight around 60 kd would be of value for clinical applications such as radioimmunolocalization and radioimmunotherapy." @default.
• W2019927307 created "2016-06-24" @default.
• W2019927307 creator A5043059264 @default.
• W2019927307 creator A5066511637 @default.
• W2019927307 creator A5075369706 @default.
• W2019927307 creator A5089533573 @default.
• W2019927307 date "2006-10-01" @default.
• W2019927307 modified "2023-09-24" @default.
• W2019927307 title "Construction and Purification of a Covalently Linked Divalent Tandem Single-Chain Fv Antibody Against Placental Alkaline Phosphatase" @default.
• W2019927307 cites W1517969155 @default.
• W2019927307 cites W1848831724 @default.
• W2019927307 cites W1964119722 @default.
• W2019927307 cites W1975097310 @default.
• W2019927307 cites W1982501635 @default.
• W2019927307 cites W1986215929 @default.
• W2019927307 cites W1996820981 @default.
• W2019927307 cites W2010039421 @default.
• W2019927307 cites W2020723775 @default.
• W2019927307 cites W2022417725 @default.
• W2019927307 cites W2040299926 @default.
• W2019927307 cites W2042359007 @default.
• W2019927307 cites W2045205653 @default.
• W2019927307 cites W2053104174 @default.
• W2019927307 cites W2069451410 @default.
• W2019927307 cites W2087939552 @default.
• W2019927307 cites W2088992922 @default.
• W2019927307 cites W2089652099 @default.
• W2019927307 cites W2095615236 @default.
• W2019927307 cites W2100837269 @default.
• W2019927307 cites W2110544075 @default.
• W2019927307 cites W2113598101 @default.
• W2019927307 cites W2115820004 @default.
• W2019927307 cites W2137651865 @default.
• W2019927307 cites W4240944597 @default.
• W2019927307 doi "https://doi.org/10.1089/hyb.2006.25.255" @default.
• W2019927307 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17044780" @default.
• W2019927307 hasPublicationYear "2006" @default.
• W2019927307 type Work @default.
• W2019927307 sameAs 2019927307 @default.
• W2019927307 citedByCount "5" @default.
• W2019927307 countsByYear W20199273072012 @default.
• W2019927307 crossrefType "journal-article" @default.
• W2019927307 hasAuthorship W2019927307A5043059264 @default.
• W2019927307 hasAuthorship W2019927307A5066511637 @default.
• W2019927307 hasAuthorship W2019927307A5075369706 @default.
• W2019927307 hasAuthorship W2019927307A5089533573 @default.
• W2019927307 hasConcept C104317684 @default.
• W2019927307 hasConcept C111919701 @default.
• W2019927307 hasConcept C116084860 @default.
• W2019927307 hasConcept C153911025 @default.
• W2019927307 hasConcept C159654299 @default.
• W2019927307 hasConcept C160160445 @default.
• W2019927307 hasConcept C178790620 @default.
• W2019927307 hasConcept C179247698 @default.
• W2019927307 hasConcept C181199279 @default.
• W2019927307 hasConcept C185592680 @default.
• W2019927307 hasConcept C203014093 @default.
• W2019927307 hasConcept C2780557392 @default.
• W2019927307 hasConcept C36394416 @default.
• W2019927307 hasConcept C40767141 @default.
• W2019927307 hasConcept C41008148 @default.
• W2019927307 hasConcept C542903549 @default.
• W2019927307 hasConcept C547475151 @default.
• W2019927307 hasConcept C55493867 @default.
• W2019927307 hasConcept C74884574 @default.
• W2019927307 hasConcept C86803240 @default.
• W2019927307 hasConceptScore W2019927307C104317684 @default.
• W2019927307 hasConceptScore W2019927307C111919701 @default.
• W2019927307 hasConceptScore W2019927307C116084860 @default.
• W2019927307 hasConceptScore W2019927307C153911025 @default.
• W2019927307 hasConceptScore W2019927307C159654299 @default.
• W2019927307 hasConceptScore W2019927307C160160445 @default.
• W2019927307 hasConceptScore W2019927307C178790620 @default.
• W2019927307 hasConceptScore W2019927307C179247698 @default.
• W2019927307 hasConceptScore W2019927307C181199279 @default.
• W2019927307 hasConceptScore W2019927307C185592680 @default.
• W2019927307 hasConceptScore W2019927307C203014093 @default.
• W2019927307 hasConceptScore W2019927307C2780557392 @default.
• W2019927307 hasConceptScore W2019927307C36394416 @default.
• W2019927307 hasConceptScore W2019927307C40767141 @default.
• W2019927307 hasConceptScore W2019927307C41008148 @default.
• W2019927307 hasConceptScore W2019927307C542903549 @default.
• W2019927307 hasConceptScore W2019927307C547475151 @default.
• W2019927307 hasConceptScore W2019927307C55493867 @default.
• W2019927307 hasConceptScore W2019927307C74884574 @default.
• W2019927307 hasConceptScore W2019927307C86803240 @default.
• W2019927307 hasIssue "5" @default.
• W2019927307 hasLocation W20199273071 @default.
• W2019927307 hasLocation W20199273072 @default.
• W2019927307 hasOpenAccess W2019927307 @default.
• W2019927307 hasPrimaryLocation W20199273071 @default.
• W2019927307 hasRelatedWork W1997382652 @default.
• W2019927307 hasRelatedWork W2009889818 @default.
• W2019927307 hasRelatedWork W2022964898 @default.
• W2019927307 hasRelatedWork W2055171409 @default.
• W2019927307 hasRelatedWork W2062588824 @default.
• W2019927307 hasRelatedWork W2068034222 @default.
• W2019927307 hasRelatedWork W2368460920 @default.

• next