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- W2019931286 abstract "The construction of nanodevices coupled with an integrated real-time detection system for evaluation of the function of biomolecules in biological processes, and enzymatic reaction kinetics occurring at the confined space or interface is a significant challenge. In this work, a nanochannel–enzyme system in which the enzymatic reaction could be investigated with an electrochemical method was constructed. The model system was established by covalently linking glucose oxidase (GOD) onto the inner wall of the nanochannels of the porous anodic alumina (PAA) membrane. An Au disc was attached at the end of the nanochannels of the PAA membrane as the working electrode for detection of H2O2 product of enzymatic reaction. The effects of ionic strength, amount of immobilized enzyme and pore diameter of the nanochannels on the enzymatic reaction kinetics were illustrated. The GOD confined in nanochannels showed high stability and reactivity. Upon addition of glucose to the nanochannel–enzyme system, the current response had a calibration range span from 0.005 to 2 mM of glucose concentration. The apparent Michaelis–Menten constant (Kmapp) of GOD confined in nanochannel was 0.4 mM. The presented work provided a platform for real-time monitoring of the enzyme reaction kinetics confined in nanospaces. Such a nanochannel–enzyme system could also help design future biosensors and enzyme reactors with high sensitivity and efficiency." @default.
- W2019931286 created "2016-06-24" @default.
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- W2019931286 date "2014-05-01" @default.
- W2019931286 modified "2023-09-27" @default.
- W2019931286 title "Enzymatic reactivity of glucose oxidase confined in nanochannels" @default.
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- W2019931286 cites W1976158697 @default.
- W2019931286 cites W1976934590 @default.
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- W2019931286 cites W1993527536 @default.
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- W2019931286 cites W2007989168 @default.
- W2019931286 cites W2008410275 @default.
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- W2019931286 doi "https://doi.org/10.1016/j.bios.2013.12.042" @default.
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