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• W2019934139 abstract "A simple, sensitive and reusable electrochemical sensor was designed for determination of mercury (II) (Hg2+) by coupling target-induced conformational switch of DNA hairpins with thymine–Hg2+–thymine (T–Hg2+–T) coordination chemistry. The hairpin probe consisted of a stem of 6 base pairs enclosing a 14 nucleotide (nt) loop and an additional 12 nt sticky end at the 3′ end. Each hairpin was labeled with ferrocene (Fc) redox tag in the middle of the loop, which was immobilized on the electrode via self-assembly of the terminal thiol moiety at the 5′ end. In the presence of target analyte, Hg2+-mediated base pairs induced the conformational change from the sticky end to open the hairpins, resulting in the ferrocene tags close to the electrode for the increasing redox current. The strong coordination reaction of T–Hg2+–T resulted in a good repeatability and intermediate precision down to 10%. The dynamic concentration range spanned from 5.0 nM to 1.0 μM Hg2+ with a detection limit of 2.5 nM at the 3sblank level. The strategy afforded exquisite selectivity for Hg2+ against other environmentally related metal ions. Inspiringly, the developed sensor could be reused by introduction of iodide (I−)." @default.
• W2019934139 created "2016-06-24" @default.
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• W2019934139 date "2013-01-01" @default.
• W2019934139 modified "2023-09-27" @default.
• W2019934139 title "Target-induced structure-switching DNA hairpins for sensitive electrochemical monitoring of mercury (II)" @default.
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• W2019934139 doi "https://doi.org/10.1016/j.bios.2012.07.015" @default.
• W2019934139 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22863115" @default.
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