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• W2019937806 abstract "An insulator is a DNA sequence that can act as a barrier to the influences of neighboring cis-acting elements, preventing gene activation, for example, when located between an enhancer and a promoter. We have identified a 42 bp fragment of the chicken beta-globin insulator that is both necessary and sufficient for enhancer blocking activity in human cells. We show that this sequence is the binding site for CTCF, a previously identified eleven-zinc finger DNA-binding protein that is highly conserved in vertebrates. CTCF sites are present in all of the vertebrate enhancer-blocking elements we have examined. We suggest that directional enhancer blocking by CTCF is a conserved component of gene regulation in vertebrates." @default.
• W2019937806 created "2016-06-24" @default.
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• W2019937806 date "1999-08-01" @default.
• W2019937806 modified "2023-10-13" @default.
• W2019937806 title "The Protein CTCF Is Required for the Enhancer Blocking Activity of Vertebrate Insulators" @default.
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• W2019937806 doi "https://doi.org/10.1016/s0092-8674(00)81967-4" @default.
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