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• W2019938899 abstract "The enzyme RNA polymerase has been partially purified from cell-free extracts of the crown-gall tumor-inducing organism Agrobacterium tumefaciens. The four triphosphates ATP, CTP, GTP, and UTP, manganese (or magnesium) ions, and DNA are all required for activity. DNA acts as a template in the formation of the new RNA molecule the base composition of which exactly mimics that of the particular DNA used. The dependence of the reaction on time, pH, and on the concentrations of nucleoside triphosphate, DNA, and protein has been worked out. The exact requirements of the entire system are delineated, the effect of physical alteration of the DNA used (heating, cooling, sonic oscillation) has been examined and a new observation made on the stimulation of DNA action by 1-minute sonic pretreatment.Actinomycin D is shown to inhibit the reaction completely at 2.8 × 10 −5 M while atabrine, a new inhibitor, requires a concentration of 3.3 × 10 −3 M under the conditions specified. Hydrolysis of the reaction product by means of a variety of procedures and other information obtained show that the reaction product is, indeed, RNA.The data reported herein are regarded as providing a satisfactory explanation for the mechanism of biosynthesis of at least one type of RNA (presumably messenger RNA) in A. tumefaciens." @default.
• W2019938899 created "2016-06-24" @default.
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• W2019938899 date "1963-01-01" @default.
• W2019938899 modified "2023-09-25" @default.
• W2019938899 title "DEOXYRIBONUCLEIC ACID DEPENDENT RIBONUCLEIC ACID SYNTHESIS IN THE CROWN-GALL TUMOR-INDUCING ORGANISM AGROBACTERIUM TUMEFACIENS" @default.
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• W2019938899 doi "https://doi.org/10.1139/y63-171" @default.
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