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- W2019938924 abstract "Intracellular pH (pHi) and several ion transport mechanisms in cultured murine salivary gland cells (SCA-9) were studied using a videomicrofluorometric method and the H+-specific probe C-SNARF-1. The aim of this study was to test SCA-9 cells' pHi regulation mechanisms and evaluate if this cell line is representative of submandibular gland cells. Resting pHi in unstimulated cells was estimated to be 7.17±0.07. To investigate the presence of Na+/H+ and Cl−/HCO3− antiports as well as Na+/K+/2Cl− symports in SCA-9 cells, we used different specific blockers, dimethyl-amiloride, disulfonic stilbene, bumetanide and furosemide. In order to study SCA-9 cell capacity to regulate their pHi in response to alkaline and acid loads, we applied the NH4Cl prepulse method to all these blockers. The results showed that SCA-9 cells possess both antiports and symports involved in pHi regulation, and that this cell line can be used as a convenient model to study pHi regulatory mechanisms in salivary cells." @default.
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- W2019938924 date "2000-02-01" @default.
- W2019938924 modified "2023-09-23" @default.
- W2019938924 title "Cultured tumor cells of murine submandibular gland origin: a model to investigate pHi regulation of salivary cells" @default.
- W2019938924 doi "https://doi.org/10.1034/j.1600-0722.2000.00670.x" @default.
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