Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019940616> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2019940616 endingPage "982" @default.
- W2019940616 startingPage "977" @default.
- W2019940616 abstract "Objective: Endothelin is thought to be related to cardiovascular disease. The purpose of this study was to determine whether endothelin levels could be reduced by a calcium antagonist and a 'natural factor'. Design: Since calcium ionophores can induce endothelin-1 messenger RNA synthesis in cultured endothelial cells, the calcium antagonist nisoldipine was used in this study to determine whether it could reduce endothelin levels. It has been reported that coculture of endothelial cells and smooth muscle cells from different species and different parts of the body can reduce endothelin levels. This study was also designed to determine whether coculture of the two cell types from the same species and the same section of an artery could reduce endothelin levels. Methods: Cultured endothelial cells from human umbilical artery (HUAEC) and umbilical vein (HUVEC) were treated with increasing concentrations of nisoldipine. HUAEC were cocultured with human umbilical artery smooth muscle cells (HUASMC). Endothelin levels were measured by a radioimmunoassay. Results: Incubation of the HUAEC with nisoldipine for either 7 or 24 h resulted in a dose-dependent (10-810-5mol/l) reduction in endothelin levels in the conditioned media. Endothelin levels in cell lysates were not detectable in either the absence or the presence of nisoldipine. This suggests that the reduction of endothelin levels in the media could be due to inhibition of endothelin synthesis. Under the same conditions, incubation of HUVEC with the same concentrations of nisoldipine produced a similar concentration-dependent reduction in endothelin levels. Endothelin levels were undetectable in the conditioned media from HUASMC. Coculture of HUAEC with HUASMC significantly reduced endothelin levels (P<0.01) compared with HUAEC cultured alone. Conclusions: Endothelin levels can be reduced by the calcium antagonist nisoldipine and a 'natural factor' associated with smooth muscle cells." @default.
- W2019940616 created "2016-06-24" @default.
- W2019940616 creator A5016102435 @default.
- W2019940616 creator A5016842679 @default.
- W2019940616 creator A5022869223 @default.
- W2019940616 creator A5030432851 @default.
- W2019940616 creator A5038217374 @default.
- W2019940616 creator A5044990471 @default.
- W2019940616 creator A5073815096 @default.
- W2019940616 date "1993-09-01" @default.
- W2019940616 modified "2023-10-16" @default.
- W2019940616 title "Reduction of endothelin levels by the dihydropyridine calcium antagonist nisoldipine and a ??natural factor?? in cultured human endothelial cells" @default.
- W2019940616 doi "https://doi.org/10.1097/00004872-199309000-00012" @default.
- W2019940616 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8254180" @default.
- W2019940616 hasPublicationYear "1993" @default.
- W2019940616 type Work @default.
- W2019940616 sameAs 2019940616 @default.
- W2019940616 citedByCount "13" @default.
- W2019940616 countsByYear W20199406162012 @default.
- W2019940616 crossrefType "journal-article" @default.
- W2019940616 hasAuthorship W2019940616A5016102435 @default.
- W2019940616 hasAuthorship W2019940616A5016842679 @default.
- W2019940616 hasAuthorship W2019940616A5022869223 @default.
- W2019940616 hasAuthorship W2019940616A5030432851 @default.
- W2019940616 hasAuthorship W2019940616A5038217374 @default.
- W2019940616 hasAuthorship W2019940616A5044990471 @default.
- W2019940616 hasAuthorship W2019940616A5073815096 @default.
- W2019940616 hasConcept C126322002 @default.
- W2019940616 hasConcept C134018914 @default.
- W2019940616 hasConcept C144980905 @default.
- W2019940616 hasConcept C149601957 @default.
- W2019940616 hasConcept C170493617 @default.
- W2019940616 hasConcept C202751555 @default.
- W2019940616 hasConcept C2776271655 @default.
- W2019940616 hasConcept C2776885963 @default.
- W2019940616 hasConcept C2777411675 @default.
- W2019940616 hasConcept C2779066535 @default.
- W2019940616 hasConcept C2909649922 @default.
- W2019940616 hasConcept C519063684 @default.
- W2019940616 hasConcept C55493867 @default.
- W2019940616 hasConcept C71924100 @default.
- W2019940616 hasConcept C86803240 @default.
- W2019940616 hasConcept C98274493 @default.
- W2019940616 hasConceptScore W2019940616C126322002 @default.
- W2019940616 hasConceptScore W2019940616C134018914 @default.
- W2019940616 hasConceptScore W2019940616C144980905 @default.
- W2019940616 hasConceptScore W2019940616C149601957 @default.
- W2019940616 hasConceptScore W2019940616C170493617 @default.
- W2019940616 hasConceptScore W2019940616C202751555 @default.
- W2019940616 hasConceptScore W2019940616C2776271655 @default.
- W2019940616 hasConceptScore W2019940616C2776885963 @default.
- W2019940616 hasConceptScore W2019940616C2777411675 @default.
- W2019940616 hasConceptScore W2019940616C2779066535 @default.
- W2019940616 hasConceptScore W2019940616C2909649922 @default.
- W2019940616 hasConceptScore W2019940616C519063684 @default.
- W2019940616 hasConceptScore W2019940616C55493867 @default.
- W2019940616 hasConceptScore W2019940616C71924100 @default.
- W2019940616 hasConceptScore W2019940616C86803240 @default.
- W2019940616 hasConceptScore W2019940616C98274493 @default.
- W2019940616 hasIssue "9" @default.
- W2019940616 hasLocation W20199406161 @default.
- W2019940616 hasLocation W20199406162 @default.
- W2019940616 hasOpenAccess W2019940616 @default.
- W2019940616 hasPrimaryLocation W20199406161 @default.
- W2019940616 hasRelatedWork W1964039040 @default.
- W2019940616 hasRelatedWork W1983371938 @default.
- W2019940616 hasRelatedWork W2002102821 @default.
- W2019940616 hasRelatedWork W2019940616 @default.
- W2019940616 hasRelatedWork W2032437128 @default.
- W2019940616 hasRelatedWork W2041469084 @default.
- W2019940616 hasRelatedWork W2095084418 @default.
- W2019940616 hasRelatedWork W2143238233 @default.
- W2019940616 hasRelatedWork W2160355439 @default.
- W2019940616 hasRelatedWork W4245472597 @default.
- W2019940616 hasVolume "11" @default.
- W2019940616 isParatext "false" @default.
- W2019940616 isRetracted "false" @default.
- W2019940616 magId "2019940616" @default.
- W2019940616 workType "article" @default.