Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019942123> ?p ?o ?g. }
- W2019942123 endingPage "927" @default.
- W2019942123 startingPage "920" @default.
- W2019942123 abstract "A gene coding for the human respiratory syncytial virus (RSV)-F protein, driven by the constitutively expressed CaMV 35S promoter, was introduced into leaf tissues of apple, Malusxdomestica Borkh. cv. Royal Gala, via Agrobacterium-mediated transformation. Two putative transgenic lines were identified, and the presence of the RSV-F gene was confirmed by polymerase chain reaction (PCR). A total of 25 plants from these different transgenic events were successfully rooted, acclimatized, and transferred to the greenhouse. Stable integration of the transgene was confirmed and transgene copy number was determined by DNA gel blot analysis. Expression of the npt-II selectable marker and RSV-F was determined using reverse-transcription polymerase chain reaction (RT-PCR). Furthermore, enzyme-linked immunosorbent assay (ELISA) revealed varying levels of protein expression of the RSV-F transgene, ranging from 0 to 20 microg/g tissue. This is a first step in an effort to assess the efficacy of using apple for developing a plant-based vaccine against RSV." @default.
- W2019942123 created "2016-06-24" @default.
- W2019942123 creator A5029662138 @default.
- W2019942123 creator A5044970802 @default.
- W2019942123 date "2010-07-01" @default.
- W2019942123 modified "2023-09-23" @default.
- W2019942123 title "Transgenic apple expressing an antigenic protein of the human respiratory syncytial virus" @default.
- W2019942123 cites W117352546 @default.
- W2019942123 cites W1273344482 @default.
- W2019942123 cites W1517361896 @default.
- W2019942123 cites W1590096533 @default.
- W2019942123 cites W1606565747 @default.
- W2019942123 cites W1705041761 @default.
- W2019942123 cites W1937512413 @default.
- W2019942123 cites W1969710187 @default.
- W2019942123 cites W1973029675 @default.
- W2019942123 cites W1980928075 @default.
- W2019942123 cites W1983410064 @default.
- W2019942123 cites W1985017143 @default.
- W2019942123 cites W1985899286 @default.
- W2019942123 cites W1989501142 @default.
- W2019942123 cites W1990719345 @default.
- W2019942123 cites W1994093841 @default.
- W2019942123 cites W1994587276 @default.
- W2019942123 cites W1995354507 @default.
- W2019942123 cites W1999075681 @default.
- W2019942123 cites W2005124447 @default.
- W2019942123 cites W2024556021 @default.
- W2019942123 cites W2033208093 @default.
- W2019942123 cites W2046045989 @default.
- W2019942123 cites W2048020967 @default.
- W2019942123 cites W2073603068 @default.
- W2019942123 cites W2078135580 @default.
- W2019942123 cites W2086975476 @default.
- W2019942123 cites W2088433600 @default.
- W2019942123 cites W2089652477 @default.
- W2019942123 cites W2091123450 @default.
- W2019942123 cites W2096628891 @default.
- W2019942123 cites W2098749611 @default.
- W2019942123 cites W2132523913 @default.
- W2019942123 cites W2133723912 @default.
- W2019942123 cites W2138265482 @default.
- W2019942123 cites W2143452171 @default.
- W2019942123 cites W2156399146 @default.
- W2019942123 cites W2322654587 @default.
- W2019942123 cites W2474897574 @default.
- W2019942123 cites W2591466108 @default.
- W2019942123 cites W3149299648 @default.
- W2019942123 cites W326463424 @default.
- W2019942123 doi "https://doi.org/10.1016/j.jplph.2010.02.003" @default.
- W2019942123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20307914" @default.
- W2019942123 hasPublicationYear "2010" @default.
- W2019942123 type Work @default.
- W2019942123 sameAs 2019942123 @default.
- W2019942123 citedByCount "16" @default.
- W2019942123 countsByYear W20199421232012 @default.
- W2019942123 countsByYear W20199421232013 @default.
- W2019942123 countsByYear W20199421232015 @default.
- W2019942123 countsByYear W20199421232016 @default.
- W2019942123 countsByYear W20199421232017 @default.
- W2019942123 countsByYear W20199421232018 @default.
- W2019942123 countsByYear W20199421232019 @default.
- W2019942123 countsByYear W20199421232022 @default.
- W2019942123 crossrefType "journal-article" @default.
- W2019942123 hasAuthorship W2019942123A5029662138 @default.
- W2019942123 hasAuthorship W2019942123A5044970802 @default.
- W2019942123 hasConcept C102230213 @default.
- W2019942123 hasConcept C104317684 @default.
- W2019942123 hasConcept C122172344 @default.
- W2019942123 hasConcept C133307689 @default.
- W2019942123 hasConcept C150194340 @default.
- W2019942123 hasConcept C153911025 @default.
- W2019942123 hasConcept C159047783 @default.
- W2019942123 hasConcept C204241405 @default.
- W2019942123 hasConcept C2522874641 @default.
- W2019942123 hasConcept C2779656422 @default.
- W2019942123 hasConcept C4692481 @default.
- W2019942123 hasConcept C49105822 @default.
- W2019942123 hasConcept C54355233 @default.
- W2019942123 hasConcept C86803240 @default.
- W2019942123 hasConceptScore W2019942123C102230213 @default.
- W2019942123 hasConceptScore W2019942123C104317684 @default.
- W2019942123 hasConceptScore W2019942123C122172344 @default.
- W2019942123 hasConceptScore W2019942123C133307689 @default.
- W2019942123 hasConceptScore W2019942123C150194340 @default.
- W2019942123 hasConceptScore W2019942123C153911025 @default.
- W2019942123 hasConceptScore W2019942123C159047783 @default.
- W2019942123 hasConceptScore W2019942123C204241405 @default.
- W2019942123 hasConceptScore W2019942123C2522874641 @default.
- W2019942123 hasConceptScore W2019942123C2779656422 @default.
- W2019942123 hasConceptScore W2019942123C4692481 @default.
- W2019942123 hasConceptScore W2019942123C49105822 @default.
- W2019942123 hasConceptScore W2019942123C54355233 @default.
- W2019942123 hasConceptScore W2019942123C86803240 @default.
- W2019942123 hasIssue "11" @default.
- W2019942123 hasLocation W20199421231 @default.
- W2019942123 hasLocation W20199421232 @default.
- W2019942123 hasOpenAccess W2019942123 @default.