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- W2019944267 abstract "SummaryRadiation inactivation of YADH in dilute aqueous solution that does not contain oxygen is due to H atoms and OH radicals, whose inactivating efficiencies are in the ratio 9 : 1. The same species are responsible for the transient absorption arising in pulse-irradiated YADH in the region of 300–320 nm, and in this case their relative efficiencies are 7·5 to 1. This resulted from steady-state and pulse radiolysis experiments using radical scavengers such as t-butanol, formate and N2O. It appears that oxygen, when present, may also contribute to the enzyme inactivation through its radiation-induced radicals. Secondary radicals originated by reaction of OH radicals with CNS−, Br− and I−, all inactivate the enzyme at a faster rate than their primary parent radical. Their relative inactivating efficiencies are of the same order as their rate constants for reaction with YADH." @default.
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- W2019944267 date "1974-01-01" @default.
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- W2019944267 title "Primary and Secondary Radicals in the Radiation-induced Inactivation of Yeast Alcoholdehydrogenase" @default.
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- W2019944267 doi "https://doi.org/10.1080/09553007414551291" @default.
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