Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019947866> ?p ?o ?g. }
- W2019947866 endingPage "214" @default.
- W2019947866 startingPage "204" @default.
- W2019947866 abstract "By expressing an array of pattern recognition receptors (PRRs), fibroblasts play an important role in stimulating and modulating the response of the innate immune system. The TLR3 ligand polyriboinosinic acid-polyribocytidylic acid, poly(I:C), a mimic of viral dsRNA, is a vaccine adjuvant candidate to activate professional antigen presenting cells (APCs). However, owing to its ligation with extracellular TLR3 on fibroblasts, subcutaneously administered poly(I:C) bears danger towards autoimmunity. It is thus in the interest of its clinical safety to deliver poly(I:C) in such a way that its activation of professional APCs is as efficacious as possible, whereas its interference with non-immune cells such as fibroblasts is controlled or even avoided. Complementary to our previous work with monocyte-derived dendritic cells (MoDCs), here we sought to control the delivery of poly(I:C) surface-assembled on microspheres to human foreskin fibroblasts (HFFs). Negatively charged polystyrene (PS) microspheres were equipped with a poly(ethylene glycol) (PEG) corona through electrostatically driven coatings with a series of polycationic poly(L-lysine)-graft-poly(ethylene glycol) copolymers, PLL-g-PEG, of varying grafting ratios g from 2.2 up to 22.7. Stable surface assembly of poly(I:C) was achieved by incubation of polymer-coated microspheres with aqueous poly(I:C) solutions. Notably, recognition of both surface-assembled and free poly(I:C) by extracellular TLR3 on HFFs halted their phagocytic activity. Ligation of surface-assembled poly(I:C) with extracellular TLR3 on HFFs could be controlled by tuning the grafting ratio g and thus the chain density of the PEG corona. When assembled on PLL-5.7-PEG-coated microspheres, poly(I:C) was blocked from triggering class I MHC molecule expression on HFFs. Secretion of interleukin (IL)-6 by HFFs after exposure to surface-assembled poly(I:C) was distinctly lower as compared to free poly(I:C). Overall, surface assembly of poly(I:C) may have potential to contribute to the clinical safety of this vaccine adjuvant candidate." @default.
- W2019947866 created "2016-06-24" @default.
- W2019947866 creator A5005539568 @default.
- W2019947866 creator A5056066010 @default.
- W2019947866 creator A5059573007 @default.
- W2019947866 creator A5067063383 @default.
- W2019947866 creator A5082085375 @default.
- W2019947866 date "2012-04-01" @default.
- W2019947866 modified "2023-10-18" @default.
- W2019947866 title "Surface assembly of poly(I:C) on PEGylated microspheres to shield from adverse interactions with fibroblasts" @default.
- W2019947866 cites W1542805665 @default.
- W2019947866 cites W1599737023 @default.
- W2019947866 cites W1603832231 @default.
- W2019947866 cites W165412956 @default.
- W2019947866 cites W1667782349 @default.
- W2019947866 cites W1777081626 @default.
- W2019947866 cites W1855316965 @default.
- W2019947866 cites W185608234 @default.
- W2019947866 cites W1917276580 @default.
- W2019947866 cites W1965917259 @default.
- W2019947866 cites W1971966571 @default.
- W2019947866 cites W1974047920 @default.
- W2019947866 cites W1975058955 @default.
- W2019947866 cites W1981969796 @default.
- W2019947866 cites W1982031623 @default.
- W2019947866 cites W1985786449 @default.
- W2019947866 cites W1987150557 @default.
- W2019947866 cites W1998211335 @default.
- W2019947866 cites W2002268461 @default.
- W2019947866 cites W2002833398 @default.
- W2019947866 cites W2006223248 @default.
- W2019947866 cites W2014732115 @default.
- W2019947866 cites W2028195000 @default.
- W2019947866 cites W2029392498 @default.
- W2019947866 cites W2030244963 @default.
- W2019947866 cites W2040775211 @default.
- W2019947866 cites W2041684789 @default.
- W2019947866 cites W2041711928 @default.
- W2019947866 cites W2045917505 @default.
- W2019947866 cites W2046183011 @default.
- W2019947866 cites W2055660637 @default.
- W2019947866 cites W2056435027 @default.
- W2019947866 cites W2057692685 @default.
- W2019947866 cites W2059532076 @default.
- W2019947866 cites W2060676927 @default.
- W2019947866 cites W2074697960 @default.
- W2019947866 cites W2076361525 @default.
- W2019947866 cites W2076398868 @default.
- W2019947866 cites W2076790209 @default.
- W2019947866 cites W2078275307 @default.
- W2019947866 cites W2088223086 @default.
- W2019947866 cites W2094233628 @default.
- W2019947866 cites W2100541792 @default.
- W2019947866 cites W2102288149 @default.
- W2019947866 cites W2115993724 @default.
- W2019947866 cites W2116097926 @default.
- W2019947866 cites W2132193957 @default.
- W2019947866 cites W2137773986 @default.
- W2019947866 cites W2158487508 @default.
- W2019947866 cites W2160063406 @default.
- W2019947866 cites W2170697062 @default.
- W2019947866 cites W2332910275 @default.
- W2019947866 cites W2614446987 @default.
- W2019947866 cites W4232170930 @default.
- W2019947866 cites W4245870928 @default.
- W2019947866 doi "https://doi.org/10.1016/j.jconrel.2012.02.002" @default.
- W2019947866 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22349184" @default.
- W2019947866 hasPublicationYear "2012" @default.
- W2019947866 type Work @default.
- W2019947866 sameAs 2019947866 @default.
- W2019947866 citedByCount "10" @default.
- W2019947866 countsByYear W20199478662012 @default.
- W2019947866 countsByYear W20199478662013 @default.
- W2019947866 countsByYear W20199478662015 @default.
- W2019947866 countsByYear W20199478662016 @default.
- W2019947866 countsByYear W20199478662019 @default.
- W2019947866 countsByYear W20199478662022 @default.
- W2019947866 crossrefType "journal-article" @default.
- W2019947866 hasAuthorship W2019947866A5005539568 @default.
- W2019947866 hasAuthorship W2019947866A5056066010 @default.
- W2019947866 hasAuthorship W2019947866A5059573007 @default.
- W2019947866 hasAuthorship W2019947866A5067063383 @default.
- W2019947866 hasAuthorship W2019947866A5082085375 @default.
- W2019947866 hasConcept C10138342 @default.
- W2019947866 hasConcept C12554922 @default.
- W2019947866 hasConcept C136449434 @default.
- W2019947866 hasConcept C153911025 @default.
- W2019947866 hasConcept C162324750 @default.
- W2019947866 hasConcept C170493617 @default.
- W2019947866 hasConcept C178790620 @default.
- W2019947866 hasConcept C185592680 @default.
- W2019947866 hasConcept C203014093 @default.
- W2019947866 hasConcept C2776709828 @default.
- W2019947866 hasConcept C2777516009 @default.
- W2019947866 hasConcept C2777863537 @default.
- W2019947866 hasConcept C2778420691 @default.
- W2019947866 hasConcept C28406088 @default.
- W2019947866 hasConcept C54400483 @default.