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• W2019948216 abstract "The purpose of this paper is to optimize an HPLC method for the determination of lycopene and β-carotene in vegetables and compare it with a spectrophotometric standard method. Among the different conditions studied the most suitable ones for our samples were: extraction with hexane/acetone/ethanol (50:25:25 v/v/v), evaporation of the hexane layer, dissolution of the dry extract in THF/ACN/methanol (15:30:55 v/v/v) and injection on a C18 column with methanol/ACN (90:10 v/v) + TEA 9 μM as mobile phase (Φ = 0.9 ml/min) and λdetection = 475 nm. Samples considered for analysis were: tomato, carrot, pepper, watermelon, persimmon and medlar. The HPLC method proposed showed adequate reproducibility (RSD < 10.5%), accuracy (100–109% recovery) and sensitive detection limits (0.6 μM for lycopene; 0.3 μM for β-carotene), with a simple preparation of the samples (one step direct extraction) and short run times (10 min) for the quantification of lycopene and β-carotene." @default.
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• W2019948216 date "2006-03-01" @default.
• W2019948216 modified "2023-10-18" @default.
• W2019948216 title "Application of a UV–vis detection-HPLC method for a rapid determination of lycopene and β-carotene in vegetables" @default.
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• W2019948216 doi "https://doi.org/10.1016/j.foodchem.2005.02.028" @default.
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