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- W2019948785 abstract "Studies were conducted using normal and human papillomavirus Type 18 (HPV-18) immortalized human keratinocytes to assess possible alterations in the differentiation process as a consequence of increased intracellular calcium concentration. Normal keratinocytes exposed to increased extracellular calcium or the phorbol ester TPA, exhibited terminal differentiation characteristics. However, late passage HPV-18 immortalized keratinocytes (designated FEP-1811) were resistant to such terminal differentiation signals. Flow cytometric analyses of 1811 cells at various stages of passage in culture revealed progressively higher levels of intracellular calcium in the immortalized cells with passage in culture when compared to normal, primary keratinocytes. Furthermore, 1811 cells isolated from tumors which developed in irradiated nude mice contained the highest level of intracellular calcium of all the cells examined. These results suggest that an increase in the concentration of intracellular calcium is associated with progression of HPV-18 immortalized keratinocytes to tumorigenicity." @default.
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- W2019948785 date "1991-05-01" @default.
- W2019948785 modified "2023-10-03" @default.
- W2019948785 title "Increased intracellular calcium is associated with progression of HPV-18 immortalized human keratinocytes to tumorigenicity" @default.
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- W2019948785 doi "https://doi.org/10.1016/0143-4160(91)90050-o" @default.
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