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• W2019949449 abstract "Clarified cell-free extracts were prepared from rapidly dividing Bacillus subtilis cells and from rabbit liver cells. These extracts were treated with [3H]-phenylmethylsulfonyl fluoride (PMSF) and analyzed by electrophoresis in isoelectric focusing polyacrylamide gels or detergent gels. Not less than 14 proteins in the B. subtilis extracts and not less than 15 proteins in rabbit liver extracts reacted covalently with PMSF. These results suggest that PMSF is not as specific for serine proteases as sometimes supposed, and its effects in physiological experiments should be interpreted with caution." @default.
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• W2019949449 date "1979-07-01" @default.
• W2019949449 modified "2023-10-16" @default.
• W2019949449 title "Specificity of the serine protease inhibitor, phenylmethylsulfonyl fluoride" @default.
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• W2019949449 doi "https://doi.org/10.1016/0006-291x(79)90653-3" @default.
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