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• W2019949900 endingPage "160" @default.
• W2019949900 startingPage "150" @default.
• W2019949900 abstract "In 1982, Rubinsky and Cravalho described a Krogh cylinder model for the analysis of cryoprotectant transport in a perfused organ. By application of the Kedem-Katchalsky equations, changes in tissue volume caused by movements of water and solute were used to predict changes in capillary radius (Cryobiology 19, 70-82, 1982). We have now measured the changes in vascular resistance that are produced when sucrose or glycerol is introduced into the perfusate flowing through rabbit kidneys at 10 degrees C, and have analyzed these data by means of the Rubinsky-Cravalho semiempirical model. The sucrose data provided an estimate of hydraulic conductivity and the dimensions of the Krogh tissue units. Three rates of addition of glycerol, 10, 30, and 90 mM/min to a final concentration of 3 M, were studied. The vascular resistance fell to approximately 40% of its initial value (radius approximately 128% of initial value) with all three rates of addition, and then returned toward its normal value while the glycerol concentration was still increasing. This behavior could be explained either by a sudden change in solute permeability at that capillary radius, or by an inverse dependence of reflection coefficient upon solute concentration. Evidence is presented that favors the latter interpretation. The best fits for the apparent hydraulic conductivity and apparent solute permeability for glycerol are 1 X 10(-6) cm/sec atm and 6 X 10(-8) cm/sec, respectively, with the reflection coefficient falling from 1.0 when the glycerol concentration is zero to 0.1 when it is 3 M. The model is used to predict tissue concentrations of glycerol throughout each experiment." @default.
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• W2019949900 date "1986-04-01" @default.
• W2019949900 modified "2023-09-24" @default.
• W2019949900 title "Analysis of the introduction and removal of glycerol in rabbit kidneys using a Krogh cylinder model" @default.
• W2019949900 cites W1974100313 @default.
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• W2019949900 doi "https://doi.org/10.1016/0011-2240(86)90006-4" @default.
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