FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019949956> ?p ?o ?g. }

• W2019949956 endingPage "339" @default.
• W2019949956 startingPage "329" @default.
• W2019949956 abstract "The objective of this research is to characterize a sodium-dependent multivitamin transporter (SMVT) in MDCK-MDR1 cells (Madin-Darby canine kidney cells transfected with the human MDR1 gene) and to investigate the feasibility of utilizing the MDCK-MDR1 cell line as an in vitro model to study the permeability of biotin-conjugated prodrugs of anti-HIV protease inhibitors. Mechanism of [3H]biotin uptake and transport was delineated. Transepithelial permeability of the biotin-conjugated prodrug, i.e., biotin-saquinavir, was also studied. Reverse transcription polymerase chain reaction (RT-PCR) was carried out to confirm the existence of SMVT in MDCK-MDR1 cells. Biotin uptake was Na+, pH, and temperature dependent, but energy independent. Uptake of biotin was found to be saturable with a Km of 13.0 μM, Vmax 21.5 of pmol min-1 (mg of protein)-1, and Kd of 0.12 μL min-1 (mg of protein)-1. Both apical and basal uptake and transepithelial transport of [3H]biotin showed that SMVT localized predominantly on the apical membrane of MDCK-MDR1 cells. [3H]Biotin uptake was inhibited by excess unlabeled biotin and its structural analogues, i.e., desthiolbiotin and valeric acid, and other vitamins such as lipoic acid and pantothenic acid, but not by acetic acid, benzoic acid, biotin methyl ester, and biocytin. Biotin-saquinavir caused lowering of [3H]biotin uptake, which indicates that it is recognized by SMVT. Apical to basal transport of [3H]biotin was also significantly inhibited in the presence of excess biotin or biotin-saquinavir. Transepithelial transport studies of biotin-saquinavir in MDCK-MDR1, wild type MDCK, and Caco-2 cells revealed that permeability of biotin-saquinavir was similar in all three cell lines. A band of SMVT mRNA at 862 bp was identified by RT-PCR. A sodium-dependent multivitamin transporter, SMVT, responsible for biotin uptake and transport, was identified and functionally characterized in MDCK-MDR1 cells. Therefore, the MDCK-MDR1 cell line may be utilized as an in vitro model to study the permeability of biotin-conjugated prodrugs such as HIV protease inhibitors. Keywords: Sodium-dependent multivitamin transporter (SMVT); MDCK-MDR1; biotin-saquinavir" @default.
• W2019949956 created "2016-06-24" @default.
• W2019949956 creator A5031328768 @default.
• W2019949956 creator A5040297353 @default.
• W2019949956 creator A5045535820 @default.
• W2019949956 creator A5058765978 @default.
• W2019949956 creator A5067470841 @default.
• W2019949956 creator A5087138805 @default.
• W2019949956 date "2006-03-25" @default.
• W2019949956 modified "2023-10-17" @default.
• W2019949956 title "Functional Characterization of Sodium-Dependent Multivitamin Transporter in MDCK-MDR1 Cells and Its Utilization as a Target for Drug Delivery" @default.
• W2019949956 cites W1552940397 @default.
• W2019949956 cites W1556464588 @default.
• W2019949956 cites W1604024471 @default.
• W2019949956 cites W1771981018 @default.
• W2019949956 cites W1900673828 @default.
• W2019949956 cites W1947844072 @default.
• W2019949956 cites W1979110203 @default.
• W2019949956 cites W1984172119 @default.
• W2019949956 cites W1985044107 @default.
• W2019949956 cites W2005692266 @default.
• W2019949956 cites W2017440345 @default.
• W2019949956 cites W2037397140 @default.
• W2019949956 cites W2049135632 @default.
• W2019949956 cites W2060191811 @default.
• W2019949956 cites W2060272169 @default.
• W2019949956 cites W2080599767 @default.
• W2019949956 cites W2082828565 @default.
• W2019949956 cites W2086395397 @default.
• W2019949956 cites W2093266223 @default.
• W2019949956 cites W2122726010 @default.
• W2019949956 cites W2124230313 @default.
• W2019949956 cites W2131241342 @default.
• W2019949956 cites W2136887031 @default.
• W2019949956 cites W2143899188 @default.
• W2019949956 cites W2173300455 @default.
• W2019949956 cites W2175645215 @default.
• W2019949956 cites W2204815678 @default.
• W2019949956 cites W2223721927 @default.
• W2019949956 cites W2404534197 @default.
• W2019949956 cites W254030193 @default.
• W2019949956 cites W255765517 @default.
• W2019949956 doi "https://doi.org/10.1021/mp0500768" @default.
• W2019949956 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2553563" @default.
• W2019949956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16749865" @default.
• W2019949956 hasPublicationYear "2006" @default.
• W2019949956 type Work @default.
• W2019949956 sameAs 2019949956 @default.
• W2019949956 citedByCount "52" @default.
• W2019949956 countsByYear W20199499562012 @default.
• W2019949956 countsByYear W20199499562013 @default.
• W2019949956 countsByYear W20199499562014 @default.
• W2019949956 countsByYear W20199499562016 @default.
• W2019949956 countsByYear W20199499562017 @default.
• W2019949956 countsByYear W20199499562018 @default.
• W2019949956 countsByYear W20199499562019 @default.
• W2019949956 countsByYear W20199499562020 @default.
• W2019949956 countsByYear W20199499562021 @default.
• W2019949956 countsByYear W20199499562022 @default.
• W2019949956 countsByYear W20199499562023 @default.
• W2019949956 crossrefType "journal-article" @default.
• W2019949956 hasAuthorship W2019949956A5031328768 @default.
• W2019949956 hasAuthorship W2019949956A5040297353 @default.
• W2019949956 hasAuthorship W2019949956A5045535820 @default.
• W2019949956 hasAuthorship W2019949956A5058765978 @default.
• W2019949956 hasAuthorship W2019949956A5067470841 @default.
• W2019949956 hasAuthorship W2019949956A5087138805 @default.
• W2019949956 hasBestOaLocation W20199499562 @default.
• W2019949956 hasConcept C153911025 @default.
• W2019949956 hasConcept C185592680 @default.
• W2019949956 hasConcept C2778204606 @default.
• W2019949956 hasConcept C55493867 @default.
• W2019949956 hasConcept C86803240 @default.
• W2019949956 hasConceptScore W2019949956C153911025 @default.
• W2019949956 hasConceptScore W2019949956C185592680 @default.
• W2019949956 hasConceptScore W2019949956C2778204606 @default.
• W2019949956 hasConceptScore W2019949956C55493867 @default.
• W2019949956 hasConceptScore W2019949956C86803240 @default.
• W2019949956 hasIssue "3" @default.
• W2019949956 hasLocation W20199499561 @default.
• W2019949956 hasLocation W20199499562 @default.
• W2019949956 hasLocation W20199499563 @default.
• W2019949956 hasLocation W20199499564 @default.
• W2019949956 hasOpenAccess W2019949956 @default.
• W2019949956 hasPrimaryLocation W20199499561 @default.
• W2019949956 hasRelatedWork W1511280725 @default.
• W2019949956 hasRelatedWork W1562086046 @default.
• W2019949956 hasRelatedWork W175320364 @default.
• W2019949956 hasRelatedWork W1969883147 @default.
• W2019949956 hasRelatedWork W1973553922 @default.
• W2019949956 hasRelatedWork W2002143759 @default.
• W2019949956 hasRelatedWork W2007813081 @default.
• W2019949956 hasRelatedWork W2008219286 @default.
• W2019949956 hasRelatedWork W2061541440 @default.
• W2019949956 hasRelatedWork W286725735 @default.
• W2019949956 hasVolume "3" @default.
• W2019949956 isParatext "false" @default.
• W2019949956 isRetracted "false" @default.

• next