FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019950422> ?p ?o ?g. }

• W2019950422 endingPage "8926" @default.
• W2019950422 startingPage "8918" @default.
• W2019950422 abstract "TrwC, the relaxase of plasmid R388, catalyzes a series of concerted DNA cleavage and strand transfer reactions on a specific site (nic) of its origin of transfer (oriT). nic contains the cleavage site and an adjacent inverted repeat (IR(2)). Mutation analysis in the nic region indicated that recognition of the IR(2) proximal arm and the nucleotides located between IR(2) and the cleavage site were essential for supercoiled DNA processing, as judged either by in vitro nic cleavage or by mobilization of a plasmid containing oriT. Formation of the IR(2) cruciform and recognition of the distal IR(2) arm and loop were not necessary for these reactions to take place. On the other hand, IR(2) was not involved in TrwC single-stranded DNA processing in vitro. For single-stranded DNA nic cleavage, TrwC recognized a sequence embracing six nucleotides upstream of the cleavage site and two nucleotides downstream. This suggests that TrwC DNA binding and cleavage are two distinguishable steps in conjugative DNA processing and that different sequence elements are recognized by TrwC in each step. IR(2)-proximal arm recognition was crucial for the initial supercoiled DNA binding. Subsequent recognition of the adjacent single-stranded DNA binding site was required to position the cleavage site in the active center of the protein so that the nic cleavage reaction could take place." @default.
• W2019950422 created "2016-06-24" @default.
• W2019950422 creator A5016151513 @default.
• W2019950422 creator A5017358738 @default.
• W2019950422 creator A5027468970 @default.
• W2019950422 creator A5048258631 @default.
• W2019950422 creator A5054646768 @default.
• W2019950422 creator A5081946504 @default.
• W2019950422 date "2010-03-01" @default.
• W2019950422 modified "2023-10-12" @default.
• W2019950422 title "Relaxase DNA Binding and Cleavage Are Two Distinguishable Steps in Conjugative DNA Processing That Involve Different Sequence Elements of the nic Site" @default.
• W2019950422 cites W126609666 @default.
• W2019950422 cites W159023385 @default.
• W2019950422 cites W1657088788 @default.
• W2019950422 cites W1966103562 @default.
• W2019950422 cites W1983668396 @default.
• W2019950422 cites W1998467050 @default.
• W2019950422 cites W2008443520 @default.
• W2019950422 cites W2018267657 @default.
• W2019950422 cites W2027250972 @default.
• W2019950422 cites W2029095216 @default.
• W2019950422 cites W2038663142 @default.
• W2019950422 cites W2044927549 @default.
• W2019950422 cites W2053976847 @default.
• W2019950422 cites W2055536494 @default.
• W2019950422 cites W2059271363 @default.
• W2019950422 cites W2062579188 @default.
• W2019950422 cites W2062929740 @default.
• W2019950422 cites W2063097994 @default.
• W2019950422 cites W2072239659 @default.
• W2019950422 cites W2087732324 @default.
• W2019950422 cites W2093848749 @default.
• W2019950422 cites W2098848381 @default.
• W2019950422 cites W2098929776 @default.
• W2019950422 cites W2101871915 @default.
• W2019950422 cites W2102824318 @default.
• W2019950422 cites W2119123521 @default.
• W2019950422 cites W2133025817 @default.
• W2019950422 cites W2136549510 @default.
• W2019950422 cites W2137874473 @default.
• W2019950422 cites W2141993375 @default.
• W2019950422 cites W2151888776 @default.
• W2019950422 cites W2154064989 @default.
• W2019950422 cites W2160039306 @default.
• W2019950422 cites W2160586883 @default.
• W2019950422 cites W2162384048 @default.
• W2019950422 cites W2164269384 @default.
• W2019950422 cites W2166429666 @default.
• W2019950422 cites W2167866277 @default.
• W2019950422 cites W2171526214 @default.
• W2019950422 doi "https://doi.org/10.1074/jbc.m109.057539" @default.
• W2019950422 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2838313" @default.
• W2019950422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20061574" @default.
• W2019950422 hasPublicationYear "2010" @default.
• W2019950422 type Work @default.
• W2019950422 sameAs 2019950422 @default.
• W2019950422 citedByCount "31" @default.
• W2019950422 countsByYear W20199504222012 @default.
• W2019950422 countsByYear W20199504222013 @default.
• W2019950422 countsByYear W20199504222014 @default.
• W2019950422 countsByYear W20199504222015 @default.
• W2019950422 countsByYear W20199504222016 @default.
• W2019950422 countsByYear W20199504222017 @default.
• W2019950422 countsByYear W20199504222018 @default.
• W2019950422 countsByYear W20199504222019 @default.
• W2019950422 countsByYear W20199504222022 @default.
• W2019950422 countsByYear W20199504222023 @default.
• W2019950422 crossrefType "journal-article" @default.
• W2019950422 hasAuthorship W2019950422A5016151513 @default.
• W2019950422 hasAuthorship W2019950422A5017358738 @default.
• W2019950422 hasAuthorship W2019950422A5027468970 @default.
• W2019950422 hasAuthorship W2019950422A5048258631 @default.
• W2019950422 hasAuthorship W2019950422A5054646768 @default.
• W2019950422 hasAuthorship W2019950422A5081946504 @default.
• W2019950422 hasBestOaLocation W20199504221 @default.
• W2019950422 hasConcept C104317684 @default.
• W2019950422 hasConcept C151730666 @default.
• W2019950422 hasConcept C153911025 @default.
• W2019950422 hasConcept C158456008 @default.
• W2019950422 hasConcept C175156509 @default.
• W2019950422 hasConcept C185592680 @default.
• W2019950422 hasConcept C43369102 @default.
• W2019950422 hasConcept C512185932 @default.
• W2019950422 hasConcept C552990157 @default.
• W2019950422 hasConcept C55493867 @default.
• W2019950422 hasConcept C71240020 @default.
• W2019950422 hasConcept C73573662 @default.
• W2019950422 hasConcept C86803240 @default.
• W2019950422 hasConceptScore W2019950422C104317684 @default.
• W2019950422 hasConceptScore W2019950422C151730666 @default.
• W2019950422 hasConceptScore W2019950422C153911025 @default.
• W2019950422 hasConceptScore W2019950422C158456008 @default.
• W2019950422 hasConceptScore W2019950422C175156509 @default.
• W2019950422 hasConceptScore W2019950422C185592680 @default.
• W2019950422 hasConceptScore W2019950422C43369102 @default.
• W2019950422 hasConceptScore W2019950422C512185932 @default.
• W2019950422 hasConceptScore W2019950422C552990157 @default.
• W2019950422 hasConceptScore W2019950422C55493867 @default.

• next