FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019953084> ?p ?o ?g. }

• W2019953084 endingPage "366" @default.
• W2019953084 startingPage "361" @default.
• W2019953084 abstract "We have used a Chinese hamster ovary cell line deficient in N-acetyl-glucosaminyltransferase 1 activity (Lec1) to study the effects of altered asparaginelinked oligosaccharides on the structure, biosynthesis, and function of glucose transporter protein. Immunoblots of membranes of Lec1 cells show a glucose transporter protein of Mr 40,000, whereas membranes of wild-type (WT) cells contain a broadly migrating Mr, 55,000 form similar to that observed in several other mammalian tissues. The total content of immunoreactive glucose transporters in Lec1 cells is 3.5-fold greater than that of WT cells. Digestion with endoglycosidases, treatment with inhibitors of glycosylation, and interactions with agarose-bound lectins demonstrate that glucose tranporters of both cell lines derive from a similar Mr 38,000 core polypeptide and that both contain asparagine-linked oligosaccharide. Transporters in Lec1 cells contain primarily “undecorated” but “trimmed” mannose-type asparagine-linked oligosaccharides, while the protein in WT cells contains a mixture of “decorated” and “trimmed” asparagine-linked oligosaccharides. Biosynthetic and turnover studies demonstrate that Lec1 cells, in contrast to WT cells, are unable fully to process the core asparagine-linked oligosaccharides of maturing glucose transporters. When radiolabeled in methionine-deficient medium both Lec1 and WT cells show similar rates of synthesis and turnover of glucose transporter proteins. It should be noted, however, that starvation for a critical amino acid may alter the ability of the cell to synthesize or degrade proteins. The abilities of Lec1 and WT cells to transport hexoses and to interact with the inhibitor cytochalasin B are very similar. The results indicate that, although altered asparagine-linked glycosylation can affect the content and biogenesis of glucose transporters, these changes do not greatly modify cellular hexose uptake. The possibility that alterations in asparagine-linked glycosylation may change the cell surface localization or acquisition of a “functional conformation” of the glucose transporter is also suggested." @default.
• W2019953084 created "2016-06-24" @default.
• W2019953084 creator A5034018460 @default.
• W2019953084 creator A5071514260 @default.
• W2019953084 creator A5076264968 @default.
• W2019953084 date "1988-08-01" @default.
• W2019953084 modified "2023-09-26" @default.
• W2019953084 title "Structure, biosynthesis, and function of the hexose transporter in Chinese hamster ovary cells deficient in N-acetylglucosaminyltransferase 1 activity" @default.
• W2019953084 cites W1482698103 @default.
• W2019953084 cites W1509312708 @default.
• W2019953084 cites W1515198637 @default.
• W2019953084 cites W1533778697 @default.
• W2019953084 cites W1537045952 @default.
• W2019953084 cites W1556927657 @default.
• W2019953084 cites W1574991890 @default.
• W2019953084 cites W1600067717 @default.
• W2019953084 cites W1966099909 @default.
• W2019953084 cites W1999841276 @default.
• W2019953084 cites W2020790836 @default.
• W2019953084 cites W2030345672 @default.
• W2019953084 cites W2033007728 @default.
• W2019953084 cites W2056917962 @default.
• W2019953084 cites W2059466822 @default.
• W2019953084 cites W2071346436 @default.
• W2019953084 cites W2074783201 @default.
• W2019953084 cites W2094400842 @default.
• W2019953084 cites W2094808389 @default.
• W2019953084 cites W2096996169 @default.
• W2019953084 cites W2100990082 @default.
• W2019953084 cites W2127483839 @default.
• W2019953084 cites W2136433521 @default.
• W2019953084 cites W2140277678 @default.
• W2019953084 cites W2152708819 @default.
• W2019953084 cites W2175041633 @default.
• W2019953084 doi "https://doi.org/10.1002/jcp.1041360221" @default.
• W2019953084 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2970467" @default.
• W2019953084 hasPublicationYear "1988" @default.
• W2019953084 type Work @default.
• W2019953084 sameAs 2019953084 @default.
• W2019953084 citedByCount "19" @default.
• W2019953084 crossrefType "journal-article" @default.
• W2019953084 hasAuthorship W2019953084A5034018460 @default.
• W2019953084 hasAuthorship W2019953084A5071514260 @default.
• W2019953084 hasAuthorship W2019953084A5076264968 @default.
• W2019953084 hasConcept C104317684 @default.
• W2019953084 hasConcept C108625454 @default.
• W2019953084 hasConcept C134018914 @default.
• W2019953084 hasConcept C1491633281 @default.
• W2019953084 hasConcept C161573976 @default.
• W2019953084 hasConcept C170493617 @default.
• W2019953084 hasConcept C175656101 @default.
• W2019953084 hasConcept C2775887612 @default.
• W2019953084 hasConcept C2775895851 @default.
• W2019953084 hasConcept C2777313579 @default.
• W2019953084 hasConcept C2779012151 @default.
• W2019953084 hasConcept C2779306644 @default.
• W2019953084 hasConcept C515207424 @default.
• W2019953084 hasConcept C54355233 @default.
• W2019953084 hasConcept C553450214 @default.
• W2019953084 hasConcept C55493867 @default.
• W2019953084 hasConcept C81885089 @default.
• W2019953084 hasConcept C86803240 @default.
• W2019953084 hasConceptScore W2019953084C104317684 @default.
• W2019953084 hasConceptScore W2019953084C108625454 @default.
• W2019953084 hasConceptScore W2019953084C134018914 @default.
• W2019953084 hasConceptScore W2019953084C1491633281 @default.
• W2019953084 hasConceptScore W2019953084C161573976 @default.
• W2019953084 hasConceptScore W2019953084C170493617 @default.
• W2019953084 hasConceptScore W2019953084C175656101 @default.
• W2019953084 hasConceptScore W2019953084C2775887612 @default.
• W2019953084 hasConceptScore W2019953084C2775895851 @default.
• W2019953084 hasConceptScore W2019953084C2777313579 @default.
• W2019953084 hasConceptScore W2019953084C2779012151 @default.
• W2019953084 hasConceptScore W2019953084C2779306644 @default.
• W2019953084 hasConceptScore W2019953084C515207424 @default.
• W2019953084 hasConceptScore W2019953084C54355233 @default.
• W2019953084 hasConceptScore W2019953084C553450214 @default.
• W2019953084 hasConceptScore W2019953084C55493867 @default.
• W2019953084 hasConceptScore W2019953084C81885089 @default.
• W2019953084 hasConceptScore W2019953084C86803240 @default.
• W2019953084 hasIssue "2" @default.
• W2019953084 hasLocation W20199530841 @default.
• W2019953084 hasLocation W20199530842 @default.
• W2019953084 hasOpenAccess W2019953084 @default.
• W2019953084 hasPrimaryLocation W20199530841 @default.
• W2019953084 hasRelatedWork W138165992 @default.
• W2019953084 hasRelatedWork W1749420422 @default.
• W2019953084 hasRelatedWork W1986541256 @default.
• W2019953084 hasRelatedWork W2008288005 @default.
• W2019953084 hasRelatedWork W2019953084 @default.
• W2019953084 hasRelatedWork W2028292452 @default.
• W2019953084 hasRelatedWork W2040518239 @default.
• W2019953084 hasRelatedWork W2073946352 @default.
• W2019953084 hasRelatedWork W2134518608 @default.
• W2019953084 hasRelatedWork W2167509949 @default.
• W2019953084 hasVolume "136" @default.
• W2019953084 isParatext "false" @default.
• W2019953084 isRetracted "false" @default.

• next