Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019955688> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2019955688 endingPage "163" @default.
- W2019955688 startingPage "159" @default.
- W2019955688 abstract "Traditionally, DNA used for PCR-based diagnostic analysis has originated from white cells fractionated from whole blood. Although this method yields substantial quantities of DNA, there are some drawbacks to the procedure, including the inconvenience of drawing blood, risk of exposure to blood-borne pathogens, liquid sample handling, and the somewhat involved extraction procedure. Alternatively, DNA for genetic diagnosis has been derived from finger stick blood samples, hair roots, cheek scrapings, and urine samples. Oral saline rinses have also been used extensively as a means of collecting buccal epithelial cells as a DNA source. However, this method still requires liquid sample handling. Herein, we present our results involving the rapid extraction of DNA from buccal cells collected on cytology brushes and swabs for use in PCR reactions, specifically the multiplex amplification of 5 exons within the CFTR gene. The quality of DNA isolated from buccal cells, collected in this manner, has been sufficient to reproducibly support multiplex amplification. Cheek cell samples and the DNA prepared from them as described here are highly stable. The success rate of PCR amplification on DNA prepared from buccal cells is 99%. In a blind study comparing the analysis of 12 mutations responsible for cystic fibrosis in multiplex products amplified with DNA from both blood and buccal cell samples from 464 individuals, there was 100% correlation of results for blood and cheek cell DNA, validating the use of DNA extracted from cheek cells collected on cytology brushes for use in genetic testing." @default.
- W2019955688 created "2016-06-24" @default.
- W2019955688 creator A5001742972 @default.
- W2019955688 creator A5011241944 @default.
- W2019955688 creator A5023085893 @default.
- W2019955688 creator A5025027807 @default.
- W2019955688 creator A5030494472 @default.
- W2019955688 creator A5033593670 @default.
- W2019955688 creator A5046006373 @default.
- W2019955688 creator A5052669092 @default.
- W2019955688 creator A5056566286 @default.
- W2019955688 date "1993-01-01" @default.
- W2019955688 modified "2023-10-18" @default.
- W2019955688 title "Multiplex PCR amplification from the CFTR gene using DNA prepared from buccal brushes/swabs" @default.
- W2019955688 doi "https://doi.org/10.1093/hmg/2.2.159" @default.
- W2019955688 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7684637" @default.
- W2019955688 hasPublicationYear "1993" @default.
- W2019955688 type Work @default.
- W2019955688 sameAs 2019955688 @default.
- W2019955688 citedByCount "381" @default.
- W2019955688 countsByYear W20199556882012 @default.
- W2019955688 countsByYear W20199556882013 @default.
- W2019955688 countsByYear W20199556882014 @default.
- W2019955688 countsByYear W20199556882015 @default.
- W2019955688 countsByYear W20199556882016 @default.
- W2019955688 countsByYear W20199556882017 @default.
- W2019955688 countsByYear W20199556882018 @default.
- W2019955688 countsByYear W20199556882019 @default.
- W2019955688 countsByYear W20199556882020 @default.
- W2019955688 countsByYear W20199556882021 @default.
- W2019955688 countsByYear W20199556882022 @default.
- W2019955688 countsByYear W20199556882023 @default.
- W2019955688 crossrefType "journal-article" @default.
- W2019955688 hasAuthorship W2019955688A5001742972 @default.
- W2019955688 hasAuthorship W2019955688A5011241944 @default.
- W2019955688 hasAuthorship W2019955688A5023085893 @default.
- W2019955688 hasAuthorship W2019955688A5025027807 @default.
- W2019955688 hasAuthorship W2019955688A5030494472 @default.
- W2019955688 hasAuthorship W2019955688A5033593670 @default.
- W2019955688 hasAuthorship W2019955688A5046006373 @default.
- W2019955688 hasAuthorship W2019955688A5052669092 @default.
- W2019955688 hasAuthorship W2019955688A5056566286 @default.
- W2019955688 hasConcept C104317684 @default.
- W2019955688 hasConcept C105702510 @default.
- W2019955688 hasConcept C153911025 @default.
- W2019955688 hasConcept C167871757 @default.
- W2019955688 hasConcept C195735259 @default.
- W2019955688 hasConcept C2781188995 @default.
- W2019955688 hasConcept C2781419344 @default.
- W2019955688 hasConcept C49105822 @default.
- W2019955688 hasConcept C54355233 @default.
- W2019955688 hasConcept C552990157 @default.
- W2019955688 hasConcept C60644358 @default.
- W2019955688 hasConcept C78063203 @default.
- W2019955688 hasConcept C86803240 @default.
- W2019955688 hasConcept C90583042 @default.
- W2019955688 hasConceptScore W2019955688C104317684 @default.
- W2019955688 hasConceptScore W2019955688C105702510 @default.
- W2019955688 hasConceptScore W2019955688C153911025 @default.
- W2019955688 hasConceptScore W2019955688C167871757 @default.
- W2019955688 hasConceptScore W2019955688C195735259 @default.
- W2019955688 hasConceptScore W2019955688C2781188995 @default.
- W2019955688 hasConceptScore W2019955688C2781419344 @default.
- W2019955688 hasConceptScore W2019955688C49105822 @default.
- W2019955688 hasConceptScore W2019955688C54355233 @default.
- W2019955688 hasConceptScore W2019955688C552990157 @default.
- W2019955688 hasConceptScore W2019955688C60644358 @default.
- W2019955688 hasConceptScore W2019955688C78063203 @default.
- W2019955688 hasConceptScore W2019955688C86803240 @default.
- W2019955688 hasConceptScore W2019955688C90583042 @default.
- W2019955688 hasIssue "2" @default.
- W2019955688 hasLocation W20199556881 @default.
- W2019955688 hasLocation W20199556882 @default.
- W2019955688 hasOpenAccess W2019955688 @default.
- W2019955688 hasPrimaryLocation W20199556881 @default.
- W2019955688 hasRelatedWork W2085664776 @default.
- W2019955688 hasRelatedWork W2090206356 @default.
- W2019955688 hasRelatedWork W2112125677 @default.
- W2019955688 hasRelatedWork W2117515889 @default.
- W2019955688 hasRelatedWork W2139947223 @default.
- W2019955688 hasRelatedWork W2287299165 @default.
- W2019955688 hasRelatedWork W2532147618 @default.
- W2019955688 hasRelatedWork W4200334082 @default.
- W2019955688 hasRelatedWork W4220896640 @default.
- W2019955688 hasRelatedWork W4303983178 @default.
- W2019955688 hasVolume "2" @default.
- W2019955688 isParatext "false" @default.
- W2019955688 isRetracted "false" @default.
- W2019955688 magId "2019955688" @default.
- W2019955688 workType "article" @default.