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- W2019963017 abstract "A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubin (UV 450 nm) and biliverdin (365 nm) was used as an analytical detector for HPLC system. An internal-surface reversed-phase silica support column was used, and the mobile phase consisted of acetonitrile: 0.5 M Tris HCl buffer (20:80, pH 7.2). Bilirubin was detected from all of the jaundiced pericardial fluid samples, and a small amount of biliverdin was detected with bilirubin in some samples. These jaundiced broilers had hepatic or bile duct lesions similar to those found in edible animals. From these results, a working definition of jaundiced broilers for poultry inspection sites was suggested: bilirubin is detectable from pericardial fluid and the carcass is in a state of yellow color change." @default.
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- W2019963017 date "1997-01-01" @default.
- W2019963017 modified "2023-10-03" @default.
- W2019963017 title "High-Performance Liquid Chromatographic Analysis of Bilirubin and Biliverdin from Jaundiced Broilers." @default.
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- W2019963017 doi "https://doi.org/10.1292/jvms.59.677" @default.
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