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- W2019964940 abstract "Abstract A rapid and sensitive colorimetric determination of deoxycytidine in urine has been described which can detect as little as 4 × 10 −3 μmole of deoxycytidine in 10 ml of urine. Deoxycytidine in urine is separated from other compounds containing 2-deoxyribose by successive treatment with cation- and anion-exchange columns. Deoxycytidine-2- 14 C is added to each sample to estimate the loss of endogenous deoxycytidine during the purification process. Average recovery of deoxycytidine is 94 ± 4%. The partially purified fraction containing deoxycytidine is hydrolyzed in 5 × 10 −3 N HCl in a boiling water bath for 130 min and the amount of 2-deoxyribose released is measured with the use of the malonaldehyde-thiobarbituric acid reaction. Under these conditions deoxycytidine is hydrolyzed completely but 20% of the 2-deoxyribose released is degraded." @default.
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- W2019964940 date "1968-05-01" @default.
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- W2019964940 title "Colorimetric analysis of deoxycytidine in urine after separation by ion-exchange column chromatography" @default.
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- W2019964940 doi "https://doi.org/10.1016/0003-2697(68)90354-0" @default.
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