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- W2019966389 abstract "The dynamic reorganization of chromatin into rigid and compact mitotic chromosomes is of fundamental importance for faithful chromosome segregation. Owing to the difficulty of investigating this process under physiological conditions, the exact morphological transitions and the molecular machinery driving chromosome condensation remain poorly defined. Here, we review how imaging-based methods can be used to quantitate chromosome condensation in vivo, focusing on yeast and animal tissue culture cells as widely used model systems. We discuss approaches how to address structural dynamics of condensing chromosomes and chromosome segments, as well as to probe for mechanical properties of mitotic chromosomes. Application of such methods to systematic perturbation studies will provide a means to reveal the molecular networks underlying the regulation of mitotic chromosome condensation." @default.
- W2019966389 created "2016-06-24" @default.
- W2019966389 creator A5038813172 @default.
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- W2019966389 date "2009-02-01" @default.
- W2019966389 modified "2023-10-14" @default.
- W2019966389 title "Assays for mitotic chromosome condensation in live yeast and mammalian cells" @default.
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- W2019966389 doi "https://doi.org/10.1007/s10577-008-9010-1" @default.
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