Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019969085> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2019969085 endingPage "858" @default.
- W2019969085 startingPage "849" @default.
- W2019969085 abstract "When plasma is filtered on Sephadex G-50, insulin immunoreactivity is recovered in two peaks. “Big” insulin, the higher molecular weight component, and “little” insulin, the lower molecular weight component, have elution volumes that correspond to those of proinsulin-125I and insulin-125I respectively. When plasma was extracted with acid ethanol and filtered in 1.0 M acetic acid, the patterns and proportions of “big” and “little” insulin were indistinguishable from those obtained by filtration of whole plasma in neutral buffer. When “big” insulin was isolated from plasma and mixed with a tracer of porcine proinsulin-125I, trypsin converted the “big” insulin immunoreactivity to the gel filtration pattern of “little” insulin in the same way that it converted the proinsulin radioactivity. More than 90% of both “big” insulin and proinsulin were converted at optimal trypsin concentrations. Our present guinea pig anti-insulin serum failed to distinguish “big” from “little” but a porcine proinsulin anti-serum, under appropriate conditions of assay, reacted strongly with “big” insulin but not at all with “little.” When tested on isolated fat cells, “little” insulin had the same bioactivity as porcine insulin, whereas “big” insulin had the same low activity as porcine proinsulin. These studies suggest that “big” insulin represents either single-chain proinsulin and/or a proinsulin intermediate that has similar low bioactivity." @default.
- W2019969085 created "2016-06-24" @default.
- W2019969085 creator A5018553093 @default.
- W2019969085 creator A5030380280 @default.
- W2019969085 creator A5062448878 @default.
- W2019969085 creator A5070064147 @default.
- W2019969085 date "1971-04-01" @default.
- W2019969085 modified "2023-10-12" @default.
- W2019969085 title "Circulating insulin: the proinsulin-like properties of “big” insulin in patients without islet cell tumors" @default.
- W2019969085 cites W1977408796 @default.
- W2019969085 cites W1990126254 @default.
- W2019969085 cites W2000184187 @default.
- W2019969085 cites W2010985011 @default.
- W2019969085 cites W2011111542 @default.
- W2019969085 cites W2041698560 @default.
- W2019969085 cites W2077413095 @default.
- W2019969085 cites W2078558764 @default.
- W2019969085 cites W2087008015 @default.
- W2019969085 cites W2093477186 @default.
- W2019969085 cites W2143873575 @default.
- W2019969085 cites W3023173633 @default.
- W2019969085 cites W3024675195 @default.
- W2019969085 cites W331502463 @default.
- W2019969085 doi "https://doi.org/10.1172/jci106556" @default.
- W2019969085 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/291999" @default.
- W2019969085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4323127" @default.
- W2019969085 hasPublicationYear "1971" @default.
- W2019969085 type Work @default.
- W2019969085 sameAs 2019969085 @default.
- W2019969085 citedByCount "27" @default.
- W2019969085 countsByYear W20199690852012 @default.
- W2019969085 countsByYear W20199690852013 @default.
- W2019969085 countsByYear W20199690852023 @default.
- W2019969085 crossrefType "journal-article" @default.
- W2019969085 hasAuthorship W2019969085A5018553093 @default.
- W2019969085 hasAuthorship W2019969085A5030380280 @default.
- W2019969085 hasAuthorship W2019969085A5062448878 @default.
- W2019969085 hasAuthorship W2019969085A5070064147 @default.
- W2019969085 hasBestOaLocation W20199690851 @default.
- W2019969085 hasConcept C126322002 @default.
- W2019969085 hasConcept C134018914 @default.
- W2019969085 hasConcept C163994747 @default.
- W2019969085 hasConcept C181199279 @default.
- W2019969085 hasConcept C185592680 @default.
- W2019969085 hasConcept C2779306644 @default.
- W2019969085 hasConcept C55493867 @default.
- W2019969085 hasConcept C71924100 @default.
- W2019969085 hasConcept C86803240 @default.
- W2019969085 hasConcept C90061646 @default.
- W2019969085 hasConceptScore W2019969085C126322002 @default.
- W2019969085 hasConceptScore W2019969085C134018914 @default.
- W2019969085 hasConceptScore W2019969085C163994747 @default.
- W2019969085 hasConceptScore W2019969085C181199279 @default.
- W2019969085 hasConceptScore W2019969085C185592680 @default.
- W2019969085 hasConceptScore W2019969085C2779306644 @default.
- W2019969085 hasConceptScore W2019969085C55493867 @default.
- W2019969085 hasConceptScore W2019969085C71924100 @default.
- W2019969085 hasConceptScore W2019969085C86803240 @default.
- W2019969085 hasConceptScore W2019969085C90061646 @default.
- W2019969085 hasIssue "4" @default.
- W2019969085 hasLocation W20199690851 @default.
- W2019969085 hasLocation W20199690852 @default.
- W2019969085 hasLocation W20199690853 @default.
- W2019969085 hasLocation W20199690854 @default.
- W2019969085 hasOpenAccess W2019969085 @default.
- W2019969085 hasPrimaryLocation W20199690851 @default.
- W2019969085 hasRelatedWork W1987930707 @default.
- W2019969085 hasRelatedWork W2029605369 @default.
- W2019969085 hasRelatedWork W2038508340 @default.
- W2019969085 hasRelatedWork W2040934062 @default.
- W2019969085 hasRelatedWork W2064751303 @default.
- W2019969085 hasRelatedWork W2068300193 @default.
- W2019969085 hasRelatedWork W2079112323 @default.
- W2019969085 hasRelatedWork W2094895170 @default.
- W2019969085 hasRelatedWork W2169847732 @default.
- W2019969085 hasRelatedWork W302189978 @default.
- W2019969085 hasVolume "50" @default.
- W2019969085 isParatext "false" @default.
- W2019969085 isRetracted "false" @default.
- W2019969085 magId "2019969085" @default.
- W2019969085 workType "article" @default.