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- W2019975133 abstract "A technique is described which permits detectionof inositol bis- and trisphos-phates (and other organic phosphates) by high-performance liquid chromatography. After separationof the compounds by anion-exchange chromatography, the column effluent is first passed through a column of immobilized alkaline phosphatase to hydrolyse the phosphate esters. Then, the stream is continuously mixed with a molybdate solution to allow detectionof the inorganic phosphate formed. The enzyme can be immobilized simply by adsorbing it to a moderately hydrophobic support. The limit of detection is less than 1 nmol. Separations are made using sodium sulfate gradients at pH 7.4 on polymeric stationary phases. Addition of calcium ions (5mM) to the mobile phase improves separation of positional isomers, and changes retention relative to adenine nucleotides which are among the main interferences in tissues." @default.
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- W2019975133 date "1986-01-01" @default.
- W2019975133 modified "2023-09-27" @default.
- W2019975133 title "Detection of inositol trisphosphate and other organic phosphates by high-performance liquid chromatography using an enzyme-loaded post-column reactor" @default.
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