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• W2019979566 abstract "Background: An intangible factor in assisted reproductive technology (ART) outcomes may be lot-to-lot variation in culture media used. The study of outcomes obtained with different batches of media may clarify the impact of this variable on the ability of cleavage stage embryos to develop appropriately in extended culture. Objective: To determine if batch-to-batch variability of extended culture media results in detectable alterations in program-wide embryo quality at the blastocyst stage. Design: IRB-approved retrospective analysis of 15 independent lots of commercially available extended culture media (IVF Science) utilized over a 15 -month period from August 2001 to October 2002. Materials and Methods: Seven hundred fifty-five patients undergoing blastocyst-stage embryo transfer following IVF were compared and evaluated on: a) conversion rate of day 3 embryos to useable blastocysts (transfer and/or cryopreservation), b) clinical pregnancy rate (CPR), and c) implantation rate. Pronuclear embryos were transferred into cleavage media 16–18 hours post-insemination, and day 3 cleavage embryos were transferred into blastocyst media 72 hours post-insemination. All patients studied received a blastocyst-stage embryo transfer. Embryology parameters were analyzed using one-way ANOVAs and clinical pregnancy and implantation rates were analyzed with complex Chi-square tests. Values are expressed as mean + SEM. Results: No differences in maternal age were observed in the groups analyzed [range 31.1 + 0.6 to 33.9 + 1.6]. The conversion rate of day 3 embryos to useable blastocysts [range 58% + 3% to 75% + 3%] did not differ consistently between the media lots. Both the CPR and implantation rates were also not affected by the media lots [range 59% + 12% to 83% + 9% and 45% + 5% to 64% + 9%, respectively]. Conclusion: The different lots of extended culture media used in this study were equally able to sustain embryo development from day 3 cleavage-stage embryos to blastocyst transfer/ cryopreservation. Batch-to-batch variability of extended culture media did not emerge as a factor impacting pregnancy outcome. ART: Laboratory Techniques" @default.
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• W2019979566 date "2004-04-01" @default.
• W2019979566 modified "2023-09-30" @default.
• W2019979566 title "Inter-batch variability of extended culture media does not result in changes in embryo quality at the blastocyst stage" @default.
• W2019979566 doi "https://doi.org/10.1016/j.fertnstert.2004.02.041" @default.
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