Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019987391> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2019987391 endingPage "152" @default.
- W2019987391 startingPage "143" @default.
- W2019987391 abstract "Ornithine and putrescine carbamoyltransferases from Streptococcus faecalis ATCC11700 have been purified and their structural properties compared. The molecular weight of native ornithine Carbamoyltransferase, measured by moleclar sieving, is 250000. It is composed of six apparently identical subunits with a molecular weight of 39000, as determined by cross-linking with the bifunctional reagent glutaraldehyde followed by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. Using the same method, putrescine Carbamoyltransferase is a trimer of 140000 consisting of three identical subunits with a molecular weight of 40000. Ornithine Carbamoyltransferase displays a narrow specificity towards its substrate, ornithine. In contrast, putrescine Carbamoyltransferase carbamoylates ornithine and several diamines (diaminopropane, diaminohexane, spermine, spermidine, cadaverine) in addition to its preferred substrate, putrescine, but with a considerably lower efficiency than for putrescine. The kinetic mechanism of putrescine Carbamoyltransferase has been investigated. Initial velocity studies yield intersecting plots using either putrescine or ornithine as substrate, indicating a sequential mechanism. The patterns of protection of the enzyme by the reactants during heat inactivation as well as the results of product and dead-end inhibition studies provide evidence for a random addition of the substrates. The putrescine inhibition that is induced by phosphate does, however, suggest that a preferred pathway exists in which carbamoylphosphate is the leading substrate. The different kinetic constants have been established. The properties of putrescine Carbamoyltransferase are compared to the known properties of other carbarnoyltransferases. The evolutionary implications of this comparison are discussed." @default.
- W2019987391 created "2016-06-24" @default.
- W2019987391 creator A5032187402 @default.
- W2019987391 creator A5037613823 @default.
- W2019987391 creator A5079849312 @default.
- W2019987391 date "1979-11-01" @default.
- W2019987391 modified "2023-10-11" @default.
- W2019987391 title "Structure and Properties of the Putrescine Carbamoyltransferase of Streptococcus faecalis" @default.
- W2019987391 cites W1491737408 @default.
- W2019987391 cites W1493925391 @default.
- W2019987391 cites W1506043065 @default.
- W2019987391 cites W1506473971 @default.
- W2019987391 cites W1510165841 @default.
- W2019987391 cites W1535175535 @default.
- W2019987391 cites W1543819219 @default.
- W2019987391 cites W1545145113 @default.
- W2019987391 cites W1557489768 @default.
- W2019987391 cites W1593688401 @default.
- W2019987391 cites W1595984863 @default.
- W2019987391 cites W1606086820 @default.
- W2019987391 cites W1625660792 @default.
- W2019987391 cites W1775749144 @default.
- W2019987391 cites W1862600675 @default.
- W2019987391 cites W1969158313 @default.
- W2019987391 cites W1977523000 @default.
- W2019987391 cites W2028686506 @default.
- W2019987391 cites W2051932436 @default.
- W2019987391 cites W2052433953 @default.
- W2019987391 cites W2054056977 @default.
- W2019987391 cites W2061170175 @default.
- W2019987391 cites W2121287972 @default.
- W2019987391 cites W2167326585 @default.
- W2019987391 cites W2332364790 @default.
- W2019987391 cites W6204808 @default.
- W2019987391 doi "https://doi.org/10.1111/j.1432-1033.1979.tb04226.x" @default.
- W2019987391 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/116850" @default.
- W2019987391 hasPublicationYear "1979" @default.
- W2019987391 type Work @default.
- W2019987391 sameAs 2019987391 @default.
- W2019987391 citedByCount "28" @default.
- W2019987391 countsByYear W20199873912012 @default.
- W2019987391 countsByYear W20199873912018 @default.
- W2019987391 countsByYear W20199873912022 @default.
- W2019987391 crossrefType "journal-article" @default.
- W2019987391 hasAuthorship W2019987391A5032187402 @default.
- W2019987391 hasAuthorship W2019987391A5037613823 @default.
- W2019987391 hasAuthorship W2019987391A5079849312 @default.
- W2019987391 hasBestOaLocation W20199873911 @default.
- W2019987391 hasConcept C181199279 @default.
- W2019987391 hasConcept C185592680 @default.
- W2019987391 hasConcept C2776796294 @default.
- W2019987391 hasConcept C2777167505 @default.
- W2019987391 hasConcept C2777468819 @default.
- W2019987391 hasConcept C2909874926 @default.
- W2019987391 hasConcept C515207424 @default.
- W2019987391 hasConcept C55493867 @default.
- W2019987391 hasConcept C71240020 @default.
- W2019987391 hasConcept C86803240 @default.
- W2019987391 hasConceptScore W2019987391C181199279 @default.
- W2019987391 hasConceptScore W2019987391C185592680 @default.
- W2019987391 hasConceptScore W2019987391C2776796294 @default.
- W2019987391 hasConceptScore W2019987391C2777167505 @default.
- W2019987391 hasConceptScore W2019987391C2777468819 @default.
- W2019987391 hasConceptScore W2019987391C2909874926 @default.
- W2019987391 hasConceptScore W2019987391C515207424 @default.
- W2019987391 hasConceptScore W2019987391C55493867 @default.
- W2019987391 hasConceptScore W2019987391C71240020 @default.
- W2019987391 hasConceptScore W2019987391C86803240 @default.
- W2019987391 hasIssue "1" @default.
- W2019987391 hasLocation W20199873911 @default.
- W2019987391 hasLocation W20199873912 @default.
- W2019987391 hasOpenAccess W2019987391 @default.
- W2019987391 hasPrimaryLocation W20199873911 @default.
- W2019987391 hasRelatedWork W1857548235 @default.
- W2019987391 hasRelatedWork W1862450636 @default.
- W2019987391 hasRelatedWork W1974304275 @default.
- W2019987391 hasRelatedWork W1977523000 @default.
- W2019987391 hasRelatedWork W1999978734 @default.
- W2019987391 hasRelatedWork W2014009260 @default.
- W2019987391 hasRelatedWork W2015931922 @default.
- W2019987391 hasRelatedWork W2031429688 @default.
- W2019987391 hasRelatedWork W2091460573 @default.
- W2019987391 hasRelatedWork W2409793846 @default.
- W2019987391 hasVolume "101" @default.
- W2019987391 isParatext "false" @default.
- W2019987391 isRetracted "false" @default.
- W2019987391 magId "2019987391" @default.
- W2019987391 workType "article" @default.