Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019994004> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2019994004 endingPage "197" @default.
- W2019994004 startingPage "193" @default.
- W2019994004 abstract "A combined promoter expression vector pBV–PAL for high-level expression of phenylalanine ammonia lyase gene of Rhodosporidium toruloides was constructed. Pal gene was cloned and inserted into the region between SalI and PstI restriction sites of expression vector pBV220 (containing PLPR promoter) to obtain recombinant expression vector pBV220–PAL. The tac promoter obtained from the plasmid pKtac was inserted into the expression vector pBV220–PAL to construct expression vector pBV–PAL. The recombinant plasmid pBV220–PAL and pBV–PAL were introduced into Escherichia coli JM109 by transformation. The result showed that the transformant E. coli JM109 (pBV–PAL) gave a much higher PAL activity than that transformant E. coli JM109 (pBV220–PAL). Recombinant PAL expression level of the transformant JM109 (pBV–PAL) was about 9.6% of total cellular protein, specific enzyme activity was 2.3-fold higher than that of the transformant JM109 (pBV220–PAL), reached 35 U/g (dry cells weight, DCW). PAL specific activity of 123 U/g (DCW) could be achieved in a 5-l fermentor. 80.5% conversion rate of trans-cinnamic acid to l-phenylalanine and 5.12 g/l l-phenylalanine were obtained after 3 h bioconversion using the transformant JM109 (pBV–PAL). The recombinant strain JM109 containing the combined promoter expression vector pBV–PAL was shown to be effective and practical to product l-phenylalanine." @default.
- W2019994004 created "2016-06-24" @default.
- W2019994004 creator A5006489984 @default.
- W2019994004 creator A5018979072 @default.
- W2019994004 creator A5027361469 @default.
- W2019994004 creator A5040247505 @default.
- W2019994004 date "2008-12-01" @default.
- W2019994004 modified "2023-09-27" @default.
- W2019994004 title "Production of l-phenylalanine from trans-cinnamic acids by high-level expression of phenylalanine ammonia lyase gene from Rhodosporidium toruloides in Escherichia coli" @default.
- W2019994004 cites W1530205158 @default.
- W2019994004 cites W1545466265 @default.
- W2019994004 cites W1589858311 @default.
- W2019994004 cites W1637747684 @default.
- W2019994004 cites W1980408397 @default.
- W2019994004 cites W1994594395 @default.
- W2019994004 cites W2017053450 @default.
- W2019994004 cites W2026031369 @default.
- W2019994004 cites W2027363017 @default.
- W2019994004 cites W2028622989 @default.
- W2019994004 cites W2046412791 @default.
- W2019994004 cites W2048310309 @default.
- W2019994004 cites W2050499271 @default.
- W2019994004 cites W2058294602 @default.
- W2019994004 cites W2071911992 @default.
- W2019994004 cites W2081382544 @default.
- W2019994004 cites W2086754463 @default.
- W2019994004 cites W4319293046 @default.
- W2019994004 doi "https://doi.org/10.1016/j.bej.2008.06.010" @default.
- W2019994004 hasPublicationYear "2008" @default.
- W2019994004 type Work @default.
- W2019994004 sameAs 2019994004 @default.
- W2019994004 citedByCount "27" @default.
- W2019994004 countsByYear W20199940042012 @default.
- W2019994004 countsByYear W20199940042013 @default.
- W2019994004 countsByYear W20199940042014 @default.
- W2019994004 countsByYear W20199940042015 @default.
- W2019994004 countsByYear W20199940042016 @default.
- W2019994004 countsByYear W20199940042017 @default.
- W2019994004 countsByYear W20199940042019 @default.
- W2019994004 countsByYear W20199940042020 @default.
- W2019994004 countsByYear W20199940042021 @default.
- W2019994004 crossrefType "journal-article" @default.
- W2019994004 hasAuthorship W2019994004A5006489984 @default.
- W2019994004 hasAuthorship W2019994004A5018979072 @default.
- W2019994004 hasAuthorship W2019994004A5027361469 @default.
- W2019994004 hasAuthorship W2019994004A5040247505 @default.
- W2019994004 hasConcept C104317684 @default.
- W2019994004 hasConcept C153911025 @default.
- W2019994004 hasConcept C185592680 @default.
- W2019994004 hasConcept C204241405 @default.
- W2019994004 hasConcept C22744801 @default.
- W2019994004 hasConcept C2777431362 @default.
- W2019994004 hasConcept C2778112864 @default.
- W2019994004 hasConcept C33161422 @default.
- W2019994004 hasConcept C40767141 @default.
- W2019994004 hasConcept C515207424 @default.
- W2019994004 hasConcept C547475151 @default.
- W2019994004 hasConcept C55493867 @default.
- W2019994004 hasConcept C86803240 @default.
- W2019994004 hasConceptScore W2019994004C104317684 @default.
- W2019994004 hasConceptScore W2019994004C153911025 @default.
- W2019994004 hasConceptScore W2019994004C185592680 @default.
- W2019994004 hasConceptScore W2019994004C204241405 @default.
- W2019994004 hasConceptScore W2019994004C22744801 @default.
- W2019994004 hasConceptScore W2019994004C2777431362 @default.
- W2019994004 hasConceptScore W2019994004C2778112864 @default.
- W2019994004 hasConceptScore W2019994004C33161422 @default.
- W2019994004 hasConceptScore W2019994004C40767141 @default.
- W2019994004 hasConceptScore W2019994004C515207424 @default.
- W2019994004 hasConceptScore W2019994004C547475151 @default.
- W2019994004 hasConceptScore W2019994004C55493867 @default.
- W2019994004 hasConceptScore W2019994004C86803240 @default.
- W2019994004 hasIssue "3" @default.
- W2019994004 hasLocation W20199940041 @default.
- W2019994004 hasOpenAccess W2019994004 @default.
- W2019994004 hasPrimaryLocation W20199940041 @default.
- W2019994004 hasRelatedWork W1496454862 @default.
- W2019994004 hasRelatedWork W2030235571 @default.
- W2019994004 hasRelatedWork W2064112457 @default.
- W2019994004 hasRelatedWork W2109992314 @default.
- W2019994004 hasRelatedWork W2348822029 @default.
- W2019994004 hasRelatedWork W2355848544 @default.
- W2019994004 hasRelatedWork W2361405022 @default.
- W2019994004 hasRelatedWork W2363386379 @default.
- W2019994004 hasRelatedWork W2392614575 @default.
- W2019994004 hasRelatedWork W2437702805 @default.
- W2019994004 hasVolume "42" @default.
- W2019994004 isParatext "false" @default.
- W2019994004 isRetracted "false" @default.
- W2019994004 magId "2019994004" @default.
- W2019994004 workType "article" @default.