FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019994282> ?p ?o ?g. }

• W2019994282 abstract "The repair and regeneration of skeletal muscle requires the action of satellite cells, which are the resident muscle stem cells. These can be isolated from human muscle biopsy samples using enzymatic digestion and their myogenic properties studied in culture. Quantitatively, the two main adherent cell types obtained from enzymatic digestion are: (i) the satellite cells (termed myogenic cells or muscle precursor cells), identified initially as CD56+ and later as CD56+/desmin+ cells and (ii) muscle-derived fibroblasts, identified as CD56– and TE-7+. Fibroblasts proliferate very efficiently in culture and in mixed cell populations these cells may overrun myogenic cells to dominate the culture. The isolation and purification of different cell types from human muscle is thus an important methodological consideration when trying to investigate the innate behavior of either cell type in culture. Here we describe a system of sorting based on the gentle enzymatic digestion of cells using collagenase and dispase followed by magnetic activated cell sorting (MACS) which gives both a high purity (>95% myogenic cells) and good yield (~2.8 x 106 ± 8.87 x 105 cells/g tissue after 7 days in vitro) for experiments in culture. This approach is based on incubating the mixed muscle-derived cell population with magnetic microbeads beads conjugated to an antibody against CD56 and then passing cells though a magnetic field. CD56+ cells bound to microbeads are retained by the field whereas CD56– cells pass unimpeded through the column. Cell suspensions from any stage of the sorting process can be plated and cultured. Following a given intervention, cell morphology, and the expression and localization of proteins including nuclear transcription factors can be quantified using immunofluorescent labeling with specific antibodies and an image processing and analysis package." @default.
• W2019994282 created "2016-06-24" @default.
• W2019994282 creator A5006940491 @default.
• W2019994282 creator A5037765310 @default.
• W2019994282 creator A5050768055 @default.
• W2019994282 creator A5068595272 @default.
• W2019994282 creator A5087645721 @default.
• W2019994282 date "2015-01-12" @default.
• W2019994282 modified "2023-10-17" @default.
• W2019994282 title "Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle" @default.
• W2019994282 cites W1275880564 @default.
• W2019994282 cites W1555460575 @default.
• W2019994282 cites W1970030059 @default.
• W2019994282 cites W1988393390 @default.
• W2019994282 cites W1991440852 @default.
• W2019994282 cites W1992400237 @default.
• W2019994282 cites W2004435199 @default.
• W2019994282 cites W2014178753 @default.
• W2019994282 cites W2017075649 @default.
• W2019994282 cites W2017985531 @default.
• W2019994282 cites W2020024177 @default.
• W2019994282 cites W2025957803 @default.
• W2019994282 cites W2042609615 @default.
• W2019994282 cites W2045038402 @default.
• W2019994282 cites W2066123991 @default.
• W2019994282 cites W2081331195 @default.
• W2019994282 cites W2084567900 @default.
• W2019994282 cites W2087803244 @default.
• W2019994282 cites W2097821935 @default.
• W2019994282 cites W2100876371 @default.
• W2019994282 cites W2105606616 @default.
• W2019994282 cites W2107416801 @default.
• W2019994282 cites W2112975464 @default.
• W2019994282 cites W2124733101 @default.
• W2019994282 cites W2138833446 @default.
• W2019994282 cites W2141201649 @default.
• W2019994282 cites W2141359943 @default.
• W2019994282 cites W2159654988 @default.
• W2019994282 cites W2163964862 @default.
• W2019994282 cites W2223742570 @default.
• W2019994282 cites W4249336316 @default.
• W2019994282 doi "https://doi.org/10.3791/52049" @default.
• W2019994282 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4354531" @default.
• W2019994282 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25650991" @default.
• W2019994282 hasPublicationYear "2015" @default.
• W2019994282 type Work @default.
• W2019994282 sameAs 2019994282 @default.
• W2019994282 citedByCount "25" @default.
• W2019994282 countsByYear W20199942822015 @default.
• W2019994282 countsByYear W20199942822016 @default.
• W2019994282 countsByYear W20199942822017 @default.
• W2019994282 countsByYear W20199942822018 @default.
• W2019994282 countsByYear W20199942822019 @default.
• W2019994282 countsByYear W20199942822020 @default.
• W2019994282 countsByYear W20199942822021 @default.
• W2019994282 countsByYear W20199942822022 @default.
• W2019994282 crossrefType "journal-article" @default.
• W2019994282 hasAuthorship W2019994282A5006940491 @default.
• W2019994282 hasAuthorship W2019994282A5037765310 @default.
• W2019994282 hasAuthorship W2019994282A5050768055 @default.
• W2019994282 hasAuthorship W2019994282A5068595272 @default.
• W2019994282 hasAuthorship W2019994282A5087645721 @default.
• W2019994282 hasBestOaLocation W20199942822 @default.
• W2019994282 hasConcept C105702510 @default.
• W2019994282 hasConcept C144024400 @default.
• W2019994282 hasConcept C147447768 @default.
• W2019994282 hasConcept C1491633281 @default.
• W2019994282 hasConcept C149923435 @default.
• W2019994282 hasConcept C153911025 @default.
• W2019994282 hasConcept C181199279 @default.
• W2019994282 hasConcept C185592680 @default.
• W2019994282 hasConcept C189014844 @default.
• W2019994282 hasConcept C203014093 @default.
• W2019994282 hasConcept C204232928 @default.
• W2019994282 hasConcept C207200792 @default.
• W2019994282 hasConcept C2778394429 @default.
• W2019994282 hasConcept C2778630268 @default.
• W2019994282 hasConcept C2779072444 @default.
• W2019994282 hasConcept C2779959927 @default.
• W2019994282 hasConcept C2780374859 @default.
• W2019994282 hasConcept C2908647359 @default.
• W2019994282 hasConcept C3763915 @default.
• W2019994282 hasConcept C54355233 @default.
• W2019994282 hasConcept C55493867 @default.
• W2019994282 hasConcept C81885089 @default.
• W2019994282 hasConcept C86803240 @default.
• W2019994282 hasConcept C95444343 @default.
• W2019994282 hasConceptScore W2019994282C105702510 @default.
• W2019994282 hasConceptScore W2019994282C144024400 @default.
• W2019994282 hasConceptScore W2019994282C147447768 @default.
• W2019994282 hasConceptScore W2019994282C1491633281 @default.
• W2019994282 hasConceptScore W2019994282C149923435 @default.
• W2019994282 hasConceptScore W2019994282C153911025 @default.
• W2019994282 hasConceptScore W2019994282C181199279 @default.
• W2019994282 hasConceptScore W2019994282C185592680 @default.
• W2019994282 hasConceptScore W2019994282C189014844 @default.
• W2019994282 hasConceptScore W2019994282C203014093 @default.
• W2019994282 hasConceptScore W2019994282C204232928 @default.
• W2019994282 hasConceptScore W2019994282C207200792 @default.
• W2019994282 hasConceptScore W2019994282C2778394429 @default.

• next