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- W2019994666 abstract "Primary cultures of human milk epithelial cells, labelled with HMFG-1, a monoclonal antibody that recognises a differentiation antigen on the membranes of breast epithelia, were separated from unlabelled cells in a fluorescence-activated cell sorter (FACS). Cells not expressing the HMFG-1 antigen have a greater in vitro growth potential than the positive ones, and give rise to cultures containing both HMFG-1 antigen positive and negative cells. The cultures derived from negative cells usually contain colonies with ‘open’ cuboidal cells and these do not express the HMFG-1 antigen. However, when they differentiate into other phenotypes such as the ‘closed’ cuboidal cell type, expression of the differentiation antigen becomes evident and the cultures then show a reduced growth potential. When milk cells are subcultured several times, a new cell type emerges which does not express the HMFG-1 antigen or the intermediate filaments typical of epithelial cells but instead express high levels of fibronectin which is found in an extracellular matrix. Whether these cells emerge by selection of an existing phenotype or by phenotypic modulation is discussed." @default.
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- W2019994666 date "1983-03-01" @default.
- W2019994666 modified "2023-09-23" @default.
- W2019994666 title "Modulation of phenotype in cultures of human milk epithelial cells and its relation to the expression of a membrane antigen" @default.
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- W2019994666 doi "https://doi.org/10.1016/0045-6039(83)90004-0" @default.
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