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• W2019996137 abstract "A piezoelectric affinity sensor has been developed to detect distinctive antigens of the human cytomegalovirus. Either the specific antibodies or the antigen were immobilized on the gold electrode. To develop a rapid immunoassay, various assay formats were tested in relation with the different antigen composition. First, a direct assay was carried out immobilizing the specific antibody on the crystal surface by passive adsorption. Next, Protein A, thiol/poly L-lysine mixed self-assembled monolayers were tested as methods of gold modification. A competitive format was exploited by immobilization of the antigen onto the crystal activated by SAM and poly L-lysine. This procedure yielded a preliminary calibration curve. A linear range between 2.5 and 5 µg/ml of gB epitope in solution and a detection limit of 1 µg/ml were measured." @default.
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• W2019996137 date "2000-11-01" @default.
• W2019996137 modified "2023-10-17" @default.
• W2019996137 title "Human cytomegalovirus detection by a quartz crystal microbalance immunosensor" @default.
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• W2019996137 doi "https://doi.org/10.1016/s0141-0229(00)00196-4" @default.
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