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• W2019998667 abstract "A voltammetric urea-sensing electrode was prepared by combining a lipid-attached urease layer with a 2,5-dihydroxythiophenol-modified gold electrode. A self-assembled monolayer of dihydroxythiophenol was prepared on the gold surface by soaking the electrode into an ethanolic solution containing the modifier. A layer of the lipid-attached enzyme and that of acetyl cellulose overcoat were successively made on the dihydroxythiophenol-modified electrode by applying a dip-coating procedure. The addition of urea in a test solution (10 mM phosphate buffer, pH 7·0) brought about an increase of pH near the urease layer. The pH shift accompanied a negative shift of the anodic peak, which corresponded to the electro-oxidation of dihydroxyphenol moiety to form quinone, on the linear sweep voltammograms for the urease/dihydroxythiophenol electrode. The concentration of urea (0·2–5 mM) could be determined by measuring the electrode current at −0·05 V versus Ag/AgCl from the voltammogram. The electrode was applied to the determination of urea in human urine: the measurement of electrode current at such a low potential provided the urea determination without any electrochemical interference from L-ascorbic acid and uric acid." @default.
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• W2019998667 date "1997-01-01" @default.
• W2019998667 modified "2023-10-18" @default.
• W2019998667 title "Voltammetric enzyme sensor for urea using mercaptohydroquinone-modified gold electrode as the base transducer" @default.
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• W2019998667 doi "https://doi.org/10.1016/s0956-5663(96)00073-5" @default.
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