Matches in SemOpenAlex for { <https://semopenalex.org/work/W2019999354> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2019999354 endingPage "68" @default.
- W2019999354 startingPage "64" @default.
- W2019999354 abstract "A complete experimental format is given for the reconstitution of human hemoglobin from the separated heme-free alpha- and beta-globin chains (alpha degrees, beta degrees) and hemin, by two alternative routes. Based on their oxygen binding properties, the reaction of the ferri-forms with reducing agent, and the response of the oxygen binding curves to pH variation and to the addition of the allosteric effector 2,3-diphosphoglycerate, the molecules are native. One reconstitution route uses direct addition of hemin to the separated globin chains with production of the separated subunits, which can then be recombined and reduced. This procedure occasions losses by precipitation in the heme-addition step except at high dilutions, and the yields are low. In the second pathway, either globin chain is mixed with the complementary untreated subunit to form the half-filled (with heme) intermediates, which combine stoichiometrically with hemin. No precipitation accompanies these reactions. For alpha-globin, the yield is about 50% because of incomplete combination with the heme-containing beta chain. For beta-globin, the yield is better than 70%. It is suggested that experiments intended to test either globin chain should use the second route in preparation for structural or functional comparisons with native hemoglobin." @default.
- W2019999354 created "2016-06-24" @default.
- W2019999354 creator A5029246162 @default.
- W2019999354 creator A5070910860 @default.
- W2019999354 creator A5084501158 @default.
- W2019999354 date "1977-01-01" @default.
- W2019999354 modified "2023-09-25" @default.
- W2019999354 title "Reconstitution of native human hemoglobin from separated globin chains and alloplex intermediates." @default.
- W2019999354 cites W1489474144 @default.
- W2019999354 cites W1500934039 @default.
- W2019999354 cites W1518938374 @default.
- W2019999354 cites W1554537480 @default.
- W2019999354 cites W1990513977 @default.
- W2019999354 cites W2006370393 @default.
- W2019999354 cites W2019758748 @default.
- W2019999354 cites W2068467565 @default.
- W2019999354 cites W2074736611 @default.
- W2019999354 cites W2087529230 @default.
- W2019999354 cites W2091911651 @default.
- W2019999354 cites W2413970802 @default.
- W2019999354 doi "https://doi.org/10.1073/pnas.74.1.64" @default.
- W2019999354 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/393197" @default.
- W2019999354 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/13370" @default.
- W2019999354 hasPublicationYear "1977" @default.
- W2019999354 type Work @default.
- W2019999354 sameAs 2019999354 @default.
- W2019999354 citedByCount "51" @default.
- W2019999354 countsByYear W20199993542012 @default.
- W2019999354 countsByYear W20199993542013 @default.
- W2019999354 countsByYear W20199993542014 @default.
- W2019999354 countsByYear W20199993542015 @default.
- W2019999354 countsByYear W20199993542016 @default.
- W2019999354 countsByYear W20199993542017 @default.
- W2019999354 countsByYear W20199993542019 @default.
- W2019999354 countsByYear W20199993542020 @default.
- W2019999354 crossrefType "journal-article" @default.
- W2019999354 hasAuthorship W2019999354A5029246162 @default.
- W2019999354 hasAuthorship W2019999354A5070910860 @default.
- W2019999354 hasAuthorship W2019999354A5084501158 @default.
- W2019999354 hasBestOaLocation W20199993541 @default.
- W2019999354 hasConcept C125996951 @default.
- W2019999354 hasConcept C134121241 @default.
- W2019999354 hasConcept C166342909 @default.
- W2019999354 hasConcept C181199279 @default.
- W2019999354 hasConcept C185592680 @default.
- W2019999354 hasConcept C191897082 @default.
- W2019999354 hasConcept C192562407 @default.
- W2019999354 hasConcept C199360897 @default.
- W2019999354 hasConcept C20705724 @default.
- W2019999354 hasConcept C2776174256 @default.
- W2019999354 hasConcept C2776217839 @default.
- W2019999354 hasConcept C2777723881 @default.
- W2019999354 hasConcept C2778917026 @default.
- W2019999354 hasConcept C41008148 @default.
- W2019999354 hasConcept C55493867 @default.
- W2019999354 hasConcept C71240020 @default.
- W2019999354 hasConceptScore W2019999354C125996951 @default.
- W2019999354 hasConceptScore W2019999354C134121241 @default.
- W2019999354 hasConceptScore W2019999354C166342909 @default.
- W2019999354 hasConceptScore W2019999354C181199279 @default.
- W2019999354 hasConceptScore W2019999354C185592680 @default.
- W2019999354 hasConceptScore W2019999354C191897082 @default.
- W2019999354 hasConceptScore W2019999354C192562407 @default.
- W2019999354 hasConceptScore W2019999354C199360897 @default.
- W2019999354 hasConceptScore W2019999354C20705724 @default.
- W2019999354 hasConceptScore W2019999354C2776174256 @default.
- W2019999354 hasConceptScore W2019999354C2776217839 @default.
- W2019999354 hasConceptScore W2019999354C2777723881 @default.
- W2019999354 hasConceptScore W2019999354C2778917026 @default.
- W2019999354 hasConceptScore W2019999354C41008148 @default.
- W2019999354 hasConceptScore W2019999354C55493867 @default.
- W2019999354 hasConceptScore W2019999354C71240020 @default.
- W2019999354 hasIssue "1" @default.
- W2019999354 hasLocation W20199993541 @default.
- W2019999354 hasLocation W20199993542 @default.
- W2019999354 hasLocation W20199993543 @default.
- W2019999354 hasLocation W20199993544 @default.
- W2019999354 hasOpenAccess W2019999354 @default.
- W2019999354 hasPrimaryLocation W20199993541 @default.
- W2019999354 hasRelatedWork W1484240844 @default.
- W2019999354 hasRelatedWork W1505502736 @default.
- W2019999354 hasRelatedWork W1999522939 @default.
- W2019999354 hasRelatedWork W2015572850 @default.
- W2019999354 hasRelatedWork W2180063892 @default.
- W2019999354 hasRelatedWork W2411647574 @default.
- W2019999354 hasRelatedWork W4289951267 @default.
- W2019999354 hasRelatedWork W83226532 @default.
- W2019999354 hasRelatedWork W957695923 @default.
- W2019999354 hasRelatedWork W2048356567 @default.
- W2019999354 hasVolume "74" @default.
- W2019999354 isParatext "false" @default.
- W2019999354 isRetracted "false" @default.
- W2019999354 magId "2019999354" @default.
- W2019999354 workType "article" @default.