FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020003786> ?p ?o ?g. }

• W2020003786 endingPage "1304" @default.
• W2020003786 startingPage "1297" @default.
• W2020003786 abstract "Abstract Using enhancers to improve the transfection efficiency of polyethylenimine (PEI) can circumvent the needs of chemical modifications as well as subsequent purification and characterization of the modified PEI. In this study, we found that incorporating trehalose into the transfection reagent could improve the transgene expression mediated by DNA‐PEI complexes. Such enhancements were not observed when trehalose was replaced by other disaccharides. In an effort to explore the mechanisms, we examined how the timing of trehalose treatments and the durations of trehalose affected the percentages of cells expressing green fluorescent protein and the levels of intracellular ethidium monoazide labeled plasmid. Treatments with trehalose for 5–120 min prior to transfection could cause drops in transfection efficiency by 30–50%; such treatments, however, hardly affected the amounts of intracellular plasmid, indicating that the preexistence of intracellular trehalose could reduce transfection efficiency without lowering the endocytic activity. The transfection efficiency remained almost unchanged when the transfected cells were treated with trehalose after the removal of transfection reagents, indicating that trehalose had minimal effects on the machinery of protein synthesis. Despite the enhanced transgene expression, the presence of trehalose during transfection showed inhibitory effects on the internalization of DNA‐PEI complexes. Additionlly, the extent of enhancement in transgene expression strongly depended on the duration of trehalose. As the above observations suggested, only during the transfection process when complexes and trehalose coexisted, trehalose became an effective enhancer of transgene expression mediated by DNA‐PEI complexes possibly by affecting the mechanisms of intracellular trafficking." @default.
• W2020003786 created "2016-06-24" @default.
• W2020003786 creator A5006026846 @default.
• W2020003786 creator A5027371586 @default.
• W2020003786 creator A5079822763 @default.
• W2020003786 creator A5088716303 @default.
• W2020003786 date "2007-01-01" @default.
• W2020003786 modified "2023-09-29" @default.
• W2020003786 title "Trehalose Enhances Transgene Expression Mediated by DNA-PEI Complexes" @default.
• W2020003786 cites W1579604458 @default.
• W2020003786 cites W1963925741 @default.
• W2020003786 cites W1972183902 @default.
• W2020003786 cites W1978828567 @default.
• W2020003786 cites W1985610066 @default.
• W2020003786 cites W1989754961 @default.
• W2020003786 cites W1994123472 @default.
• W2020003786 cites W2010655741 @default.
• W2020003786 cites W2023532191 @default.
• W2020003786 cites W2032179220 @default.
• W2020003786 cites W2033299624 @default.
• W2020003786 cites W2036354853 @default.
• W2020003786 cites W2041999173 @default.
• W2020003786 cites W2043170269 @default.
• W2020003786 cites W2050606485 @default.
• W2020003786 cites W2055020116 @default.
• W2020003786 cites W2057587268 @default.
• W2020003786 cites W2060823909 @default.
• W2020003786 cites W2067855092 @default.
• W2020003786 cites W2071914105 @default.
• W2020003786 cites W2072545910 @default.
• W2020003786 cites W2074870430 @default.
• W2020003786 cites W2078722426 @default.
• W2020003786 cites W2078810655 @default.
• W2020003786 cites W2079657322 @default.
• W2020003786 cites W2094793265 @default.
• W2020003786 cites W2108605550 @default.
• W2020003786 cites W2130639648 @default.
• W2020003786 cites W2154329741 @default.
• W2020003786 cites W2167812052 @default.
• W2020003786 cites W2188802704 @default.
• W2020003786 cites W2327041436 @default.
• W2020003786 doi "https://doi.org/10.1021/bp070224m" @default.
• W2020003786 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17927205" @default.
• W2020003786 hasPublicationYear "2007" @default.
• W2020003786 type Work @default.
• W2020003786 sameAs 2020003786 @default.
• W2020003786 citedByCount "8" @default.
• W2020003786 countsByYear W20200037862012 @default.
• W2020003786 countsByYear W20200037862013 @default.
• W2020003786 countsByYear W20200037862015 @default.
• W2020003786 countsByYear W20200037862018 @default.
• W2020003786 crossrefType "journal-article" @default.
• W2020003786 hasAuthorship W2020003786A5006026846 @default.
• W2020003786 hasAuthorship W2020003786A5027371586 @default.
• W2020003786 hasAuthorship W2020003786A5079822763 @default.
• W2020003786 hasAuthorship W2020003786A5088716303 @default.
• W2020003786 hasConcept C102230213 @default.
• W2020003786 hasConcept C104317684 @default.
• W2020003786 hasConcept C153911025 @default.
• W2020003786 hasConcept C185592680 @default.
• W2020003786 hasConcept C22744801 @default.
• W2020003786 hasConcept C2775925850 @default.
• W2020003786 hasConcept C2778452849 @default.
• W2020003786 hasConcept C54009773 @default.
• W2020003786 hasConcept C552990157 @default.
• W2020003786 hasConcept C55493867 @default.
• W2020003786 hasConcept C79879829 @default.
• W2020003786 hasConcept C86803240 @default.
• W2020003786 hasConcept C95444343 @default.
• W2020003786 hasConceptScore W2020003786C102230213 @default.
• W2020003786 hasConceptScore W2020003786C104317684 @default.
• W2020003786 hasConceptScore W2020003786C153911025 @default.
• W2020003786 hasConceptScore W2020003786C185592680 @default.
• W2020003786 hasConceptScore W2020003786C22744801 @default.
• W2020003786 hasConceptScore W2020003786C2775925850 @default.
• W2020003786 hasConceptScore W2020003786C2778452849 @default.
• W2020003786 hasConceptScore W2020003786C54009773 @default.
• W2020003786 hasConceptScore W2020003786C552990157 @default.
• W2020003786 hasConceptScore W2020003786C55493867 @default.
• W2020003786 hasConceptScore W2020003786C79879829 @default.
• W2020003786 hasConceptScore W2020003786C86803240 @default.
• W2020003786 hasConceptScore W2020003786C95444343 @default.
• W2020003786 hasIssue "6" @default.
• W2020003786 hasLocation W20200037861 @default.
• W2020003786 hasLocation W20200037862 @default.
• W2020003786 hasOpenAccess W2020003786 @default.
• W2020003786 hasPrimaryLocation W20200037861 @default.
• W2020003786 hasRelatedWork W2072526150 @default.
• W2020003786 hasRelatedWork W2085373224 @default.
• W2020003786 hasRelatedWork W2095149823 @default.
• W2020003786 hasRelatedWork W2318761112 @default.
• W2020003786 hasRelatedWork W2365306427 @default.
• W2020003786 hasRelatedWork W2379167648 @default.
• W2020003786 hasRelatedWork W2393224696 @default.
• W2020003786 hasRelatedWork W2410054136 @default.
• W2020003786 hasRelatedWork W2787086553 @default.
• W2020003786 hasRelatedWork W3083875150 @default.
• W2020003786 hasVolume "23" @default.

• next