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• W2020023009 abstract "The kinetics of NH4+, the assimilation of NH4+ and nitrate uptake by Catenella nipae (Rhodophyta) were compared with Ulva lactuca (Chlorophyta). Both algal species demonstrated saturable NH4+ and nitrate uptake kinetics. Uptake of NH3 by simple diffusion across the plasmalemma could not account for the observed saturation uptake kinetics of ammonia-N (NH3+NH4+), so NH4+ was the chemical form being taken up by the transport systems of the cells. Although the Vmax of NH4+ uptake by C. nipae and U. lactuca was high (≈550 and 450 μmol g−1 DW h−1, respectively), the Km for U. lactuca (≈85 μM) was much lower than that for C. nipae (≈692 μM). The Km and Vmax values for nitrate uptake were much lower than for NH4+ for both C. nipae (Km≈5 μM; Vmax≈8.3 μmol g−1 DW h−1) and U. lactuca (Km≈34 μM; Vmax≈116 μmol g−1 DW h−1). Over the incubation times used (up to 28 min) there was no apparent induction of nitrate transport in either species. There was no evidence for induction of NH4+ transport in C. nipae but incubation time did affect the kinetics of NH4+ uptake in U. lactuca. At high concentrations of NH4+, U. lactuca rapidly assimilated it into organic N with limited build-up of intracellular NH4+ whereas C. nipae accumulated large amounts of NH4+ because uptake of NH4+ overtook the rate of assimilation. The effects of species-specific differences and experimental design on uptake-kinetic estimates are discussed in the light of the results of this other comparable studies. C. nipae is promising as a bioindicator species of the N-status of estuaries but U. lactuca changes its N-status too quickly for it to be a useful bioindicator of environmental conditions." @default.
• W2020023009 created "2016-06-24" @default.
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• W2020023009 date "2003-06-01" @default.
• W2020023009 modified "2023-09-29" @default.
• W2020023009 title "Uptake kinetics and assimilation of inorganic nitrogen by Catenella nipae and Ulva lactuca" @default.
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• W2020023009 doi "https://doi.org/10.1016/s0304-3770(03)00037-8" @default.
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