FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020032810> ?p ?o ?g. }

• W2020032810 endingPage "70" @default.
• W2020032810 startingPage "61" @default.
• W2020032810 abstract "Adeno-associated virus (AAV) is making its place in gene therapy applications; however, the industry is still facing obstacles in producing a large quantity of highly purified material for clinical studies. Insect cell technology can be used to produce AAV to meet the current demand. During the purification process it was observed that there was a reduced recovery of AAV produced in insect cells, Spodoptera frugiperda (Sf9). It was assumed that the formation of AAV agglomerates and the interaction of AAV with other cellular components were major contributors to this loss. After studying different systems of extraction a sequence of treatment for primary recovery of AAV from cell paste was developed. This sequence was necessary to reduce the AAV losses and to increase the recovery. The purification method avoided the use of ultracentrifugation and adopted chromatographic methods for the purification of AAV. Primary recovery, ion exchange chromatography and hydrophobic interaction chromatography gave an overall yield of 75% from the extracted AAV. The purification process was based on chromatographic methods; therefore, it can be scaled up. Although this method was developed for AAV type 2, it is believed that this method could be modified easily to purify other AAV serotypes." @default.
• W2020032810 created "2016-06-24" @default.
• W2020032810 creator A5023781129 @default.
• W2020032810 creator A5069297606 @default.
• W2020032810 creator A5074303933 @default.
• W2020032810 date "2007-01-01" @default.
• W2020032810 modified "2023-10-07" @default.
• W2020032810 title "Primary recovery and chromatographic purification of adeno-associated virus type 2 produced by baculovirus/insect cell system" @default.
• W2020032810 cites W1511668063 @default.
• W2020032810 cites W1554727410 @default.
• W2020032810 cites W1560333061 @default.
• W2020032810 cites W1567485512 @default.
• W2020032810 cites W1567696028 @default.
• W2020032810 cites W1968382713 @default.
• W2020032810 cites W1983903557 @default.
• W2020032810 cites W1987990463 @default.
• W2020032810 cites W1988597673 @default.
• W2020032810 cites W1988624696 @default.
• W2020032810 cites W1996388379 @default.
• W2020032810 cites W2001657410 @default.
• W2020032810 cites W2010484367 @default.
• W2020032810 cites W2017608179 @default.
• W2020032810 cites W2021345893 @default.
• W2020032810 cites W2037175789 @default.
• W2020032810 cites W2042959864 @default.
• W2020032810 cites W2048008983 @default.
• W2020032810 cites W2049386436 @default.
• W2020032810 cites W2051159468 @default.
• W2020032810 cites W2059108053 @default.
• W2020032810 cites W2062249345 @default.
• W2020032810 cites W2067866007 @default.
• W2020032810 cites W2079090019 @default.
• W2020032810 cites W2081694771 @default.
• W2020032810 cites W2087020558 @default.
• W2020032810 cites W2088763985 @default.
• W2020032810 cites W2093830879 @default.
• W2020032810 cites W2115185064 @default.
• W2020032810 cites W2118338200 @default.
• W2020032810 cites W2128298711 @default.
• W2020032810 cites W2139668172 @default.
• W2020032810 cites W2151017330 @default.
• W2020032810 cites W2154329712 @default.
• W2020032810 cites W2158987899 @default.
• W2020032810 cites W2174590326 @default.
• W2020032810 cites W2180590412 @default.
• W2020032810 cites W2202185794 @default.
• W2020032810 cites W2532840516 @default.
• W2020032810 cites W3105570083 @default.
• W2020032810 doi "https://doi.org/10.1016/j.jviromet.2006.09.011" @default.
• W2020032810 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17055590" @default.
• W2020032810 hasPublicationYear "2007" @default.
• W2020032810 type Work @default.
• W2020032810 sameAs 2020032810 @default.
• W2020032810 citedByCount "42" @default.
• W2020032810 countsByYear W20200328102012 @default.
• W2020032810 countsByYear W20200328102014 @default.
• W2020032810 countsByYear W20200328102015 @default.
• W2020032810 countsByYear W20200328102016 @default.
• W2020032810 countsByYear W20200328102017 @default.
• W2020032810 countsByYear W20200328102018 @default.
• W2020032810 countsByYear W20200328102019 @default.
• W2020032810 countsByYear W20200328102020 @default.
• W2020032810 countsByYear W20200328102021 @default.
• W2020032810 countsByYear W20200328102022 @default.
• W2020032810 countsByYear W20200328102023 @default.
• W2020032810 crossrefType "journal-article" @default.
• W2020032810 hasAuthorship W2020032810A5023781129 @default.
• W2020032810 hasAuthorship W2020032810A5069297606 @default.
• W2020032810 hasAuthorship W2020032810A5074303933 @default.
• W2020032810 hasConcept C103878061 @default.
• W2020032810 hasConcept C104317684 @default.
• W2020032810 hasConcept C159047783 @default.
• W2020032810 hasConcept C185592680 @default.
• W2020032810 hasConcept C206967267 @default.
• W2020032810 hasConcept C2522874641 @default.
• W2020032810 hasConcept C2777212814 @default.
• W2020032810 hasConcept C2777773057 @default.
• W2020032810 hasConcept C2778107364 @default.
• W2020032810 hasConcept C40767141 @default.
• W2020032810 hasConcept C43617362 @default.
• W2020032810 hasConcept C54689828 @default.
• W2020032810 hasConcept C55493867 @default.
• W2020032810 hasConcept C86803240 @default.
• W2020032810 hasConcept C8985478 @default.
• W2020032810 hasConcept C92087593 @default.
• W2020032810 hasConceptScore W2020032810C103878061 @default.
• W2020032810 hasConceptScore W2020032810C104317684 @default.
• W2020032810 hasConceptScore W2020032810C159047783 @default.
• W2020032810 hasConceptScore W2020032810C185592680 @default.
• W2020032810 hasConceptScore W2020032810C206967267 @default.
• W2020032810 hasConceptScore W2020032810C2522874641 @default.
• W2020032810 hasConceptScore W2020032810C2777212814 @default.
• W2020032810 hasConceptScore W2020032810C2777773057 @default.
• W2020032810 hasConceptScore W2020032810C2778107364 @default.
• W2020032810 hasConceptScore W2020032810C40767141 @default.
• W2020032810 hasConceptScore W2020032810C43617362 @default.
• W2020032810 hasConceptScore W2020032810C54689828 @default.
• W2020032810 hasConceptScore W2020032810C55493867 @default.

• next