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• W2020054871 abstract "To develop a protocol for MALDI (matrix-assisted laser desorption ionization) imaging mass spectrometry for mapping the distributions of alpha-crystallin and its modified forms in human lens tissue as a function of lens age and cataract.Frozen human lenses were cryosectioned equatorially and axially into 20-mum-thick sections, and the sections were mounted onto conductive glass slides by methanol soft-landing. An ethanol washing procedure facilitated uniform matrix crystal formation by a two-step matrix deposition procedure to produce high-quality mass spectral data. Molecular images of modified and unmodified alpha-crystallin subunits were obtained from mass spectral data acquired in 100-mum steps across normal and cataractous lens sections. Proteins extracted from the lens sections were digested with endoproteinase Glu-C and subjected to mass spectrometric analysis for identification of modifications.Intact alpha-crystallin signals were detected primarily in the outer cortical fiber cells in lenses up to 29 years of age. Multiple truncation products were observed for alpha-crystallin that increased in abundance, both with distance into the lens and with lens age. Phosphorylated alphaB-crystallin forms were most abundant in the cortical region of older lenses. In axial sections, no significant anterior-posterior pole variation was observed. A previously unreported alphaA-crystallin mutation was detected in an age-matched cataractous human lens.A method has been developed to spatially map the age-related changes of human lens alpha-crystallin by MALDI imaging mass spectrometry including a novel L52F alphaA-crystallin mutation in a cataractous lens. Application of this spatially resolved proteomic technique to lens biology enhances the understanding of alpha-crystallin protein processing in aging and diseased human lenses." @default.
• W2020054871 created "2016-06-24" @default.
• W2020054871 creator A5043823252 @default.
• W2020054871 creator A5075009221 @default.
• W2020054871 date "2009-09-01" @default.
• W2020054871 modified "2023-10-14" @default.
• W2020054871 title "Age-Related Changes in the Spatial Distribution of Human Lens α-Crystallin Products by MALDI Imaging Mass Spectrometry" @default.
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• W2020054871 doi "https://doi.org/10.1167/iovs.09-3522" @default.
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