FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020056656> ?p ?o ?g. }

• W2020056656 endingPage "4826" @default.
• W2020056656 startingPage "4818" @default.
• W2020056656 abstract "Transfer RNA (tRNA) contains a number of complex ‘hypermodified’ nucleosides that are essential for a number of genetic processes. Intermediate forms of these nucleosides are rarely found in tRNA despite the fact that modification is not generally a complete process. We propose that the modification machinery is tuned into an efficient ‘assembly line’ that performs the modification steps at similar, or sequentially increasing, rates to avoid build-up of possibly deleterious intermediates. To investigate this concept, we measured steady-state kinetics for the final two steps of the biosynthesis of the mnm5s2U nucleoside in Escherichia coli tRNAGlu, which are both catalysed by the bifunctional MnmC enzyme. High-performance liquid chromatography-based assays using selectively under-modified tRNA substrates gave a Km value of 600 nM and kcat 0.34 s−1 for the first step, and Km 70 nM and kcat 0.31 s−1 for the second step. These values show that the second reaction occurs faster than the first reaction, or at a similar rate at very high substrate concentrations. This result indicates that the enzyme is kinetically tuned to produce fully modified mnm5(s2)U while avoiding build-up of the nm5(s2)U intermediate. The assay method developed here represents a general approach for the comparative analysis of tRNA-modifying enzymes." @default.
• W2020056656 created "2016-06-24" @default.
• W2020056656 creator A5008213992 @default.
• W2020056656 creator A5026032684 @default.
• W2020056656 date "2011-02-08" @default.
• W2020056656 modified "2023-09-27" @default.
• W2020056656 title "Assay of both activities of the bifunctional tRNA-modifying enzyme MnmC reveals a kinetic basis for selective full modification of cmnm5s2U to mnm5s2U" @default.
• W2020056656 cites W1482562661 @default.
• W2020056656 cites W1546767835 @default.
• W2020056656 cites W1963570235 @default.
• W2020056656 cites W1973290333 @default.
• W2020056656 cites W1973806960 @default.
• W2020056656 cites W1986316905 @default.
• W2020056656 cites W1995314607 @default.
• W2020056656 cites W2000390805 @default.
• W2020056656 cites W2011057627 @default.
• W2020056656 cites W2029080366 @default.
• W2020056656 cites W2036588330 @default.
• W2020056656 cites W2037577397 @default.
• W2020056656 cites W2039081525 @default.
• W2020056656 cites W2052024371 @default.
• W2020056656 cites W2056752459 @default.
• W2020056656 cites W2062189579 @default.
• W2020056656 cites W2071462258 @default.
• W2020056656 cites W2074400862 @default.
• W2020056656 cites W2083171274 @default.
• W2020056656 cites W2092579362 @default.
• W2020056656 cites W2104440526 @default.
• W2020056656 cites W2110850678 @default.
• W2020056656 cites W2116801182 @default.
• W2020056656 cites W2120276342 @default.
• W2020056656 cites W2122324193 @default.
• W2020056656 cites W2142652402 @default.
• W2020056656 cites W2154073658 @default.
• W2020056656 cites W2167144660 @default.
• W2020056656 cites W2168281180 @default.
• W2020056656 cites W2171593351 @default.
• W2020056656 doi "https://doi.org/10.1093/nar/gkr071" @default.
• W2020056656 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3113582" @default.
• W2020056656 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21306992" @default.
• W2020056656 hasPublicationYear "2011" @default.
• W2020056656 type Work @default.
• W2020056656 sameAs 2020056656 @default.
• W2020056656 citedByCount "13" @default.
• W2020056656 countsByYear W20200566562012 @default.
• W2020056656 countsByYear W20200566562013 @default.
• W2020056656 countsByYear W20200566562014 @default.
• W2020056656 countsByYear W20200566562016 @default.
• W2020056656 countsByYear W20200566562017 @default.
• W2020056656 countsByYear W20200566562019 @default.
• W2020056656 countsByYear W20200566562023 @default.
• W2020056656 crossrefType "journal-article" @default.
• W2020056656 hasAuthorship W2020056656A5008213992 @default.
• W2020056656 hasAuthorship W2020056656A5026032684 @default.
• W2020056656 hasBestOaLocation W20200566561 @default.
• W2020056656 hasConcept C104317684 @default.
• W2020056656 hasConcept C121332964 @default.
• W2020056656 hasConcept C148898269 @default.
• W2020056656 hasConcept C153957851 @default.
• W2020056656 hasConcept C161790260 @default.
• W2020056656 hasConcept C181199279 @default.
• W2020056656 hasConcept C185592680 @default.
• W2020056656 hasConcept C18903297 @default.
• W2020056656 hasConcept C2776543447 @default.
• W2020056656 hasConcept C2777289219 @default.
• W2020056656 hasConcept C2780443040 @default.
• W2020056656 hasConcept C41183919 @default.
• W2020056656 hasConcept C547475151 @default.
• W2020056656 hasConcept C55493867 @default.
• W2020056656 hasConcept C56856141 @default.
• W2020056656 hasConcept C58431802 @default.
• W2020056656 hasConcept C62520636 @default.
• W2020056656 hasConcept C67705224 @default.
• W2020056656 hasConcept C71240020 @default.
• W2020056656 hasConcept C86803240 @default.
• W2020056656 hasConceptScore W2020056656C104317684 @default.
• W2020056656 hasConceptScore W2020056656C121332964 @default.
• W2020056656 hasConceptScore W2020056656C148898269 @default.
• W2020056656 hasConceptScore W2020056656C153957851 @default.
• W2020056656 hasConceptScore W2020056656C161790260 @default.
• W2020056656 hasConceptScore W2020056656C181199279 @default.
• W2020056656 hasConceptScore W2020056656C185592680 @default.
• W2020056656 hasConceptScore W2020056656C18903297 @default.
• W2020056656 hasConceptScore W2020056656C2776543447 @default.
• W2020056656 hasConceptScore W2020056656C2777289219 @default.
• W2020056656 hasConceptScore W2020056656C2780443040 @default.
• W2020056656 hasConceptScore W2020056656C41183919 @default.
• W2020056656 hasConceptScore W2020056656C547475151 @default.
• W2020056656 hasConceptScore W2020056656C55493867 @default.
• W2020056656 hasConceptScore W2020056656C56856141 @default.
• W2020056656 hasConceptScore W2020056656C58431802 @default.
• W2020056656 hasConceptScore W2020056656C62520636 @default.
• W2020056656 hasConceptScore W2020056656C67705224 @default.
• W2020056656 hasConceptScore W2020056656C71240020 @default.
• W2020056656 hasConceptScore W2020056656C86803240 @default.
• W2020056656 hasIssue "11" @default.
• W2020056656 hasLocation W20200566561 @default.
• W2020056656 hasLocation W20200566562 @default.

• next