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- W2020072050 abstract "A technique for efficient cytochalasin-induced enucleation was used to prepare karyoplasts--nuclei surrounded by a thin shell of cytoplasm and an outer cell membrane. Methods for estimating the quantity of cytoplasm remaining in karyoplasts indicated that they contained less than 10% of the amount found in whole cells. Procedures for separating karyoplasts from contaminating cytoplasmic fragments and whole cells are also described. Freshly prepared L-cell karyoplasts were unable to adhere to and spread upon a surface. However, after incubation for several days, about 30% of the karyoplasts regained these abilities to some degree. A portion of the regenerating karyoplasts were then observed to divide. These events were confirmed and recorded by time-lapse cinematography. In addition, by culturing karyoplasts under appropriate conditions, clones were isolated." @default.
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- W2020072050 date "1976-01-01" @default.
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- W2020072050 title "The regeneration and division of mouse L-cell karyoplasts" @default.
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- W2020072050 doi "https://doi.org/10.1016/0092-8674(76)90261-0" @default.
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